Planta Med 2008; 74(12): 1446-1450
DOI: 10.1055/s-2008-1081352
Pharmacology
Original Paper
© Georg Thieme Verlag KG Stuttgart · New York

Inhibitory Effect of Panduratin A on UV-induced Activation of Mitogen-Activated Protein Kinases (MAPKs) in Dermal Fibroblast Cells

Jae-Seok Shim1 , Yi-Young Kwon2 , Young-Sun Han2 , Jae-Kwan Hwang1 , 2
  • 1Department of Biomaterials Science and Engineering, Yonsei University, Seoul, Korea
  • 2Department of Biotechnology, Yonsei University, Seoul, Korea
Weitere Informationen

Publikationsverlauf

Received: April 23, 2008 Revised: July 4, 2008

Accepted: July 9, 2008

Publikationsdatum:
05. August 2008 (online)

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Abstract

Exposure of the skin to ultraviolet (UV) induces photoaging associated with up-regulated matrix metalloproteinases (MMPs) activities and decreased collagen synthesis. We previously reported that panduratin A, a chalcone compound isolated from Kaempferia pandurata Roxb., decreased MMP-1 expression in UV-irradiated human skin fibroblasts. Here, we have investigated the effect of panduratin A on UV-induced activation of mitogen-activated protein kinases (MAPKs) signaling modules such as extracellular-regulated protein kinase (ERK), Jun-N-terminal kinase (JNK) and p38 kinase. Treatment with panduratin A in the range of 0.001 – 0.1 μM significantly inhibited UV-induced ERK, JNK and p38 activation. Moreover, inhibition of ERK, JNK and p38 by panduratin A resulted in decreased c-Fos expression and c-Jun phosphorylation induced by UV, which led to inhibition of activator protein-1 (AP-1) DNA binding activity. Panduratin A showed stronger activity than epigallocatechin 3-O-gallate (EGCG) known as a natural anti-aging agent. The results suggest that panduratin A can down-regulate UV-induced MMP-1 expression by inhibiting the MAPKs pathways and AP-1 activation.

Abbreviations

AP-1:activator protein-1

EGCG:epigallocatechin 3-O-gallate

ERK:extracellular-regulated protein kinase

JNK:c-Jun N-terminal kinase

MAPK:mitogen-activated protein kinase

MMP:matrix metalloproteinase

UV:ultraviolet

References

Prof. Jae-Kwan Hwang

Department of Biotechnology

Yonsei University

134 Shinchon-dong

Seodaemun-gu

Seoul 120–749

Korea

Telefon: +82-2-2123-5881

Fax: +82-2-362-7265

eMail: jkhwang@yonsei.ac.kr