Activated thrombin-activatable fibrinolysis inhibitor attenuates spontaneous fibrinolysis of batroxobin-induced fibrin deposition in rat lungs

Chengliang Wu; Ningzheng Dong; Valdeci da Cunha; Baby Martin-McNulty; Katherine Tran; Mariko Nagashima; Qingyu Wu; John Morser; Yi-Xin Wang

doi:10.1160/TH02-09-0104

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2003; 90(03): 414-421
DOI: 10.1160/TH02-09-0104

Blood Coagulation, Fibrinolysis and Cellular Haemostasis

Schattauer GmbH

Activated thrombin-activatable fibrinolysis inhibitor attenuates spontaneous fibrinolysis of batroxobin-induced fibrin deposition in rat lungs

Chengliang Wu

¹Departments of Cardiovascular Research and Pharmacology, Berlex Biosciences, Richmond, California, USA

²Jiangsu Institute of Hematology, Suzhou University, Suzhou, P.R. China

,

Ningzheng Dong

¹Departments of Cardiovascular Research and Pharmacology, Berlex Biosciences, Richmond, California, USA

²Jiangsu Institute of Hematology, Suzhou University, Suzhou, P.R. China

,

Valdeci da Cunha

¹Departments of Cardiovascular Research and Pharmacology, Berlex Biosciences, Richmond, California, USA

,

Baby Martin-McNulty

¹Departments of Cardiovascular Research and Pharmacology, Berlex Biosciences, Richmond, California, USA

,

Katherine Tran

¹Departments of Cardiovascular Research and Pharmacology, Berlex Biosciences, Richmond, California, USA

,

Mariko Nagashima

¹Departments of Cardiovascular Research and Pharmacology, Berlex Biosciences, Richmond, California, USA

,

Qingyu Wu

¹Departments of Cardiovascular Research and Pharmacology, Berlex Biosciences, Richmond, California, USA

,

John Morser

¹Departments of Cardiovascular Research and Pharmacology, Berlex Biosciences, Richmond, California, USA

,

Yi-Xin Wang

¹Departments of Cardiovascular Research and Pharmacology, Berlex Biosciences, Richmond, California, USA

› Author Affiliations

Abstract

Summary

Studies have shown that inhibition of TAFI by small peptides enhances pharmacological effects of tPA in animal models of thrombosis, suggesting that TAFI modulates the fibrinolytic system. In this study, we investigated the effect of activated human TAFI (TAFIa) on endogenous fibrinolysis in a rat model of intravascular fibrin deposition. ¹²⁵I-labeled fibrinogen was injected intravenously followed by a bolus injection of batroxo-bin, a thrombin-like enzyme. Batroxobin cleaved fibrinogen to form insoluble fibrin that was deposited in tissues, including the lungs. This was shown by a decrease of radioactivity in the blood as a result of consumption of ¹²⁵I-labeled fibrinogen and an elevation of radioactivity in the lungs 5 min following batroxobin administration. Endogenous fibrinolysis was detected by a gradual increase in radioactivity in the blood and a decrease in radioactivity in the lungs at 30 min, an indication of radio-labeled fibrin degradation products (FDPs) being released into the circulation from the tissues. Intravenous administration of human TAFIa dose-dependently attenuated the later phase reduction of radioactivity in the lungs. When the dose of TAFIa was 218 μg/kg, giving a peak plasma level of TAFIa 0.9 ± 0.05 μg/ml, the spontaneous fibrinolysis was completely prevented. These results provide direct evidence that an increase in circulating TAFIa impairs endogenous clot lysis in a rat model of fibrin deposition.

Keywords

TAFI - PAI-1 - batroxobin - fibrinolysis - rat - lung fibrin deposition

Full Text

References

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