Soluble CD146, a novel endothelial marker, is increased in physiopathological settings linked to endothelial junctional alteration

Nathalie Bardin; Valérie Moal; Francine Anfosso; Laurent Daniel; Philippe Brunet; José Sampol; Françoise Dignat George

doi:10.1160/TH02-11-0285

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2003; 90(05): 915-920
DOI: 10.1160/TH02-11-0285

Vascular Development and Vessel Remodeling

Schattauer GmbH

Soluble CD146, a novel endothelial marker, is increased in physiopathological settings linked to endothelial junctional alteration

Nathalie Bardin

¹INSERM EMI 0019, Physiopathologie de l’endothélium, Université de la Méditerranée, UFR Pharmacie, Marseille, France

,

Valérie Moal

²Service de Néphrologie, Hôpital de la Conception, Marseille, France

,

Francine Anfosso

¹INSERM EMI 0019, Physiopathologie de l’endothélium, Université de la Méditerranée, UFR Pharmacie, Marseille, France

,

Laurent Daniel

³EA 3281, Faculté de Médecine, Marseille, France

,

Philippe Brunet

¹INSERM EMI 0019, Physiopathologie de l’endothélium, Université de la Méditerranée, UFR Pharmacie, Marseille, France

³EA 3281, Faculté de Médecine, Marseille, France

,

José Sampol

¹INSERM EMI 0019, Physiopathologie de l’endothélium, Université de la Méditerranée, UFR Pharmacie, Marseille, France

,

Françoise Dignat George

¹INSERM EMI 0019, Physiopathologie de l’endothélium, Université de la Méditerranée, UFR Pharmacie, Marseille, France

› Author Affiliations

Abstract

Summary

CD146, a novel cell adhesion molecule localized at the endothelial junction, is involved in the control of cell-cell cohesion. It is found as a soluble form in conditioned medium of cultured endothelial cells. We developed an ELISA and report for the first time the presence of a soluble form of CD146 (sCD146) in the plasma of healthy subjects. Mean sCD146 values (260 ± 60 ng/ml) were higher in subjects over 50 years and in men. We therefore investigated sCD146 values in patients with chronic renal failure (CRF), a clinical setting associated with junctional alterations. A significant increase in sCD146 was found in patients with CRF matched with controls (457 ± 181 ng/ml versus 288 ± 82 ng/ml respectively, p<0.0001). This increase was corroborated by increased endothelial expression of CD146 on kidney biopsies from patients with CRF. In contrast, in patients with CRF no modulation was observed for the soluble and cell-associated form of CD31, another junctional molecule. Together these data indicate that sCD146 circulates in the plasma of healthy subjects. Modifications of its basal levels could reflect alterations of junctional functions such as vascular permeability.

Keywords

CD146 - endothelial cells - endothelial junction - soluble endothelial marker - chronic renal failure

Full Text

References

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