Shedding of active tissue factor by aortic smooth muscle cells (SMCs) undergoing apoptosis

Anne-Cécile Brisset; Anne-Dominique Terrisse; Dominique Dupouy; Lise Tellier; Stéphane Pech; Chantal Navarro; Pierre Sié

doi:10.1160/TH02-12-0291

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 2003; 90(03): 511-518
DOI: 10.1160/TH02-12-0291

Vascular Development and Vessel Remodelling

Schattauer GmbH

Shedding of active tissue factor by aortic smooth muscle cells (SMCs) undergoing apoptosis

Anne-Cécile Brisset

¹EA 2049 Laboratoire de Recherche sur l’Hémostase et la Thrombose, Université Paul Sabatier, Toulouse, France

,

Anne-Dominique Terrisse

¹EA 2049 Laboratoire de Recherche sur l’Hémostase et la Thrombose, Université Paul Sabatier, Toulouse, France

,

Dominique Dupouy

¹EA 2049 Laboratoire de Recherche sur l’Hémostase et la Thrombose, Université Paul Sabatier, Toulouse, France

,

Lise Tellier

¹EA 2049 Laboratoire de Recherche sur l’Hémostase et la Thrombose, Université Paul Sabatier, Toulouse, France

,

Stéphane Pech

¹EA 2049 Laboratoire de Recherche sur l’Hémostase et la Thrombose, Université Paul Sabatier, Toulouse, France

,

Chantal Navarro

¹EA 2049 Laboratoire de Recherche sur l’Hémostase et la Thrombose, Université Paul Sabatier, Toulouse, France

,

Pierre Sié

¹EA 2049 Laboratoire de Recherche sur l’Hémostase et la Thrombose, Université Paul Sabatier, Toulouse, France

› Institutsangaben

Abstract

Summary

As apoptosis of neo-intimal SMCs is a feature of advanced atherosclerotic plaques, the procoagulant properties of SMCs of synthetic phenotype undergoing apoptosis were investigated. SMCs isolated from rat aorta obtained 10 days after balloon injury, previously found to up-regulate Tissue Factor (TF) and Tissue Factor Inhibitor (TFPI) and to release large amounts of TFPI (Ghrib et al. Thromb Haemost 2002;87:1043-50), were sensitive to the apoptosis induced by Fas-ligand. During this process, surface TF activity rose by a factor 10 over 6 hours, in parallel with a proportional increase in prothrombinase, while TF protein expressed at the membrane significantly decreased. The microparticles (MPs) produced during SMC death bore intact and functional TF, but the release of TFPI did not change, so that the balance shifted to a procoagulant state during apoptosis. Shed MPs enhanced thrombus formation in flowing whole blood over collagen coated-glass slides. Apoptotic SMCs in atherosclerotic plaques represent a reservoir of highly thrombogenic material, released into the blood stream in case of spontaneous or mechanical plaque disruption.

Keywords

Apoptosis - arterial thrombosis - smooth muscle cells - tissue factor

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Referenzen

References
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