Plasminogen activator inhibitor-1 and outcome after femoropopliteal angioplasty: analysis of genotype and plasma levels

Wolfgang Mlekusch; Martin Schillinger; Markus Exner; Schila Sabeti; Christine Mannhalter; Erich Minar; Oswald Wagner

doi:10.1160/TH03-03-0159

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2003; 90(04): 717-723
DOI: 10.1160/TH03-03-0159

Vascular Development and Vessel Remodelling

Schattauer GmbH

Plasminogen activator inhibitor-1 and outcome after femoropopliteal angioplasty: analysis of genotype and plasma levels

Wolfgang Mlekusch

¹Department of Angiology Chemical Laboratory Diagnostics, University of Vienna, Medical School, Vienna, Austria

,

Martin Schillinger

¹Department of Angiology Chemical Laboratory Diagnostics, University of Vienna, Medical School, Vienna, Austria

,

Markus Exner

²Department of Medical and Chemical Laboratory Diagnostics, University of Vienna, Medical School, Vienna, Austria

,

Schila Sabeti

¹Department of Angiology Chemical Laboratory Diagnostics, University of Vienna, Medical School, Vienna, Austria

,

Christine Mannhalter

²Department of Medical and Chemical Laboratory Diagnostics, University of Vienna, Medical School, Vienna, Austria

,

Erich Minar

¹Department of Angiology Chemical Laboratory Diagnostics, University of Vienna, Medical School, Vienna, Austria

,

Oswald Wagner

²Department of Medical and Chemical Laboratory Diagnostics, University of Vienna, Medical School, Vienna, Austria

› Author Affiliations

Abstract

Summary

Plasminogen activator inhibitor-1 (PAI-1) is suggested to be involved in the pathophysiology of early thrombosis and late restenosis after percutaneous transluminal angioplasty (PTA). The role of the PAI-1 promoter genotype in this context is indeterminate. We investigated the association of the (4G/5G) polymorphism at nucleotide position (–675) in the PAI-1 gene promoter, PAI-1 plasma levels, and postangioplasty outcome after femoropopliteal PTA.

We studied 251 consecutive patients who underwent femoropopliteal PTA. In a subgroup of 86 patients PAI-1 plasma levels at baseline,8,24 and 48 hours postintervention were measured and correlated to the genotype. Patients were followed for early thrombosis and the late restenosis (≥50%) within 12 months. Multivariate Cox proportional hazards analysis was performed to assess the association between the PAI-1 genotype and PTA failure.

Results show that the PAI-1 genotype was neither associated with PAI-1 plasma levels (p=0.40) nor the change of PAI-1 from baseline to 8 (p=0.39), 24 (p=0.86) and 48 hours (p=0.89). Three out of 35 homozygous (4G/4G) patients (9%) had early thrombotic reocclusions, compared to two out of 153 heterozygous (4G/5G) patients (1%) and none of the 63 homozygous (5G/5G) patients (p=0.007). Restenosis after median 5 months (interquartile range 3 to 9) was found in 117 patients (42%), without significant association between the PAI-1 genotype and late postangioplasty failure (Log Rank p=0.95).

We can conclude that carriers of the 4G allele exhibited a higher frequency of early thrombotic reocclusions after percutaneous angioplasty. However, the PAI-1 gene promoter polymorphism (4G/5G) was not associated with PAI-1 plasma levels or late postangioplasty restenosis.

Keywords

Gene expression - intravascular devices - proteases/inhibitors - restenosis

Full Text

References

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