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DOI: 10.1160/TH03-05-0287
Extracellular fibrinogen binding protein, Efb, from Staphylococcus aureus binds to platelets and inhibits platelet aggregation
Financial support: This work was supported by grants from the Swedish Research Council (K2001-16X-12218-05C) and Biostapro AB.Publikationsverlauf
Received
13. Mai 2003
Accepted after revision
06. Januar 2004
Publikationsdatum:
06. Dezember 2017 (online)
![](https://www.thieme-connect.de/media/10.1055-s-00035024/200404/lookinside/thumbnails/10-1160-th03-05-0287-1.jpg)
Summary
S. aureus produces and secretes a protein, extracellular fibrinogen binding protein (Efb), which contributes to virulence in wound infection. We have shown here that Efb is a potent inhibitor of platelet aggregation. Efb can bind specifically to platelets by two mechanisms; 1) to fibrinogen naturally bound to the surface of activated platelets and 2) also directly to a surface localized component on the platelets. This latter binding of Efb is independent of fibrinogen. The specific binding of Efb to the putative receptor on the platelet surface results in a stimulated, non-functional binding of fibrinogen in a dose dependent manner, distinct from natural binding of fibrinogen to platelets. The natural binding of fibrinogen to GPIIb/IIIa on activated platelets could be blocked by a monoclonal antibody against this integrin, whereas the Efb-mediated fibrinogen binding could not be blocked. The enhanced Efb-dependent fibrinogen binding to platelets is of a nature that does not promote aggregation of the platelets; instead it inhibits aggregation. The anti-thrombotic action of Efb may explain the effect of Efb on wound healing, which is delayed in the presence of Efb.
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