Sustained elevated amounts of circulating procoagulant membrane microparticles and soluble GPV after acute myocardial infarction in diabetes mellitus

Olivier Morel; Bénédicte Hugel; Laurence Jesel; François Lanza; Marie-Pierre Douchet; Michel Zupan; Michel Chauvin; Jean-Pierre Cazenave; Jean-Marie Freyssinet; Florence Toti

doi:10.1160/TH03-05-0294

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2004; 91(02): 345-353
DOI: 10.1160/TH03-05-0294

Platelets and Blood Cells

Schattauer GmbH

Sustained elevated amounts of circulating procoagulant membrane microparticles and soluble GPV after acute myocardial infarction in diabetes mellitus

Authors

Olivier Morel

¹Fédération de Cardiologie des Hôpitaux Universitaires de Strasbourg

²Institut d’Hématologie et d’Immunologie, Université Louis Pasteur, Strasbourg
Bénédicte Hugel

²Institut d’Hématologie et d’Immunologie, Université Louis Pasteur, Strasbourg
Laurence Jesel

¹Fédération de Cardiologie des Hôpitaux Universitaires de Strasbourg
François Lanza

³Etablissement Français du Sang-Alsace

⁴U. 311 INSERM, Strasbourg
Marie-Pierre Douchet

¹Fédération de Cardiologie des Hôpitaux Universitaires de Strasbourg
Michel Zupan

¹Fédération de Cardiologie des Hôpitaux Universitaires de Strasbourg
Michel Chauvin

¹Fédération de Cardiologie des Hôpitaux Universitaires de Strasbourg
Jean-Pierre Cazenave

³Etablissement Français du Sang-Alsace

⁴U. 311 INSERM, Strasbourg
Jean-Marie Freyssinet

²Institut d’Hématologie et d’Immunologie, Université Louis Pasteur, Strasbourg

⁵U. 143 INSERM, Hôpital de Bicêtre
Florence Toti

²Institut d’Hématologie et d’Immunologie, Université Louis Pasteur, Strasbourg

⁵U. 143 INSERM, Hôpital de Bicêtre

⁶Faculté de Médecine Paris-Sud, Le Kremlin-Bicêtre, France

Abstract

Summary

During myocardial infarction (MI), platelet activation and endothelial apoptosis are responsible for the release of procoagulant membrane-derived microparticles (MP) in the blood flow. MP prothrombotic and proinflammatory properties may be crucial for coronary prognosis. Elevated amounts of circulating procoagulant MP were described in diabetes mellitus (DM), and could be of particular significance in a MI context. We evaluated the prothrombotic status of DM and non-DM (NDM) patients at days 1 and 6 after MI, by measurement of circulating procoagulant MP and soluble GPV (sGPV), the platelet glycoprotein V major fragment released upon thrombin cleavage. Variations were compared to values measured in healthy volunteers (HV). Procoagulant MP were captured onto insolubilized annexin V and quantified by prothrombinase assay. Their cellular origin was assessed. With respect to HV, the levels of procoagulant MP detected at D1 and D6 were elevated in DM and NDM, MP being significantly higher in DM vs. NDM. The high amounts of platelet-derived MP and the correlation between procoagulant MP and sGPV, testify to the central role of thrombin-activated platelets during MI in both DM and NDM subsets. The release of platelet and endothelial cell-derived MP persisted at D6 and was more important in DM, the associated prothrombotic risk being also reflected by higher levels of sGPV. The endothelial damage revealed by endothelial-derived MP was twice that observed in NDM patients. In DM patients presenting cardiovascular events at 6 month follow-up, MP levels were significantly higher at D1 after MI than in those without complication (24.9 ± 4.8 vs. 12.3 ± 2.7 nM PhtdSer, p = 0.02), suggesting a prognostic potential for MP.

Keywords

Apoptosis - thrombosis - atherosclerosis - endothelium damage - platelet stimulation - sCD40 Ligand

Full Text

References

References
1 Aupeix K, Hugel B, Martin T. et al. The significance of shed membrane particles during programmed cell death in vitro, and in vivo, in HIV-1 infection. J Clin Invest 1997; 99: 1546-54.
2 VanWijk MJ, VanBavel E, Sturk A, Nieuwland R. Microparticles in cardiovascular diseases. Cardiovasc Res 2003; 59: 277-87.
3 Mallat Z, Benamer H, Hugel B. et al. Elevated levels of shed membrane microparticles with procoagulant potential in the peripheral circulating blood of patients with acute coronary syndromes. Circulation 2000; 101: 841-3.
4 Mallat Z, Tedgui A. Current perspective on the role of apoptosis in atherothrombotic disease. Circ Res 2001; 88: 998-1003.
5 Fukumoto H, Naito Z, Asano G, Aramaki T. Immunohistochemical and morphometric evaluations of coronary atherosclerotic plaques associated with myocardial infarction and diabetes mellitus. J Atheroscler Thromb 1998; 05: 29-35.
6 Farb A, Burke AP, Tang AL. et al. Coronary plaque erosion without rupture into a lipid core. A frequent cause of coronary thrombosis in sudden coronary death. Circulation 1996; 93: 1354-63.
7 Zwaal RF, Schroit AJ. Pathophysiologic implications of membrane phospholipid assymetry in blood cells. Blood 1997; 89: 1121-32.
8 Rauch U, Nemerson Y. Tissue factor, the blood, and the arterial wall. Trends Cardiovasc Med 2000; 10: 139-43.
9 Bach R, Rifkin DB. Expression of tissue factor procoagulant activity: regulation by cytosolic calcium. Proc Natl Acad Sci U S A 1990; 87: 6995-9.
10 Barry OP, FitzGerald GA. Mechanisms of cellular activation by platelet microparticles. Thromb Haemost 1999; 82: 794-800.
11 Freyssinet JM, Toti F, Hugel B. et al. Apoptosis in vascular disease. Thromb Haemost 1999; 82: 727-35.
12 Sabatier F, Darmon P, Hugel B. et al. Type 1 and type 2 diabetic patients display different patterns of cellular microparticles. Diabetes 2002; 51: 2840-5.
13 Omoto S, Nomura S, Shouzu A. et al. Detection of monocyte-derived microparticles in patients with Type II diabetes mellitus. Diabetologia 2002; 45: 550-5.
14 Frustaci A, Kajstura J, Chimenti C. et al. Myocardial cell death in human diabetes. Circ Res 2000; 87: 1123-32.
15 Cai L, Li W, Wang G. et al. Hyperglycemiainduced apoptosis in mouse myocardium: mitochondrial cytochrome C-mediated caspase-3 activation pathway. Diabetes 2002; 51: 1938-48.
16 Ravanat C, Freund M, Mangin P. et al. GPV is a marker of in vivo platelet activation—study in a rat thrombosis model. Thromb Haemost 2000; 83: 327-33.
17 Slupsky JR, Kalbas M, Willuweit A. et al. Activated platelets induce tissue factor expression on human umbilical vein endothelial cells by ligation of CD40. Thromb Haemost 1998; 80: 1008-14.
18 Varo N, de Lemos JA, Libby P. et al. Soluble CD40L: risk prediction after acute coronary syndromes. Circulation 2003; 108: 1049-52.
19 Jacobson SH, Egberg N, Hylander B. et al. Correlation between soluble markers of endothelial dysfunction in patients with renal failure. Am J Nephrol 2002; 22: 42-7.
20 Gawaz M, Neumann FJ, Ott I. et al. Platelet function in acute myocardial infarction treated with direct angioplasty. Circulation 1996; 93: 229-37.
21 Ardissino D, Merlini PA, Bauer KA. et al. Thrombogenic potential of human coronary atherosclerotic plaques. Blood 2001; 98: 2726-9.
22 Mallat Z, Hugel B, Ohan J. et al. Shed membrane microparticles with procoagulant potential in human atherosclerotic plaques: a role for apoptosis in plaque thrombogenicity. Circulation 1999; 99: 348-53.
23 Hugel B, Socie G, Vu T. et al. Elevated levels of circulating procoagulant microparticles in patients with paroxysmal nocturnal hemoglobinuria and aplastic anemia. Blood 1999; 93: 3451-6.
24 Rossig L, Hoffmann J, Hugel B. et al. Vitamin C inhibits endothelial cell apoptosis in congestive heart failure. Circulation 2001; 104: 2182-7.
25 Azorsa DO, Moog S, Ravanat C. et al. Measurement of GPV released by activated platelets using a sensitive immunocapture ELISA—its use to follow platelet storage in transfusion. Thromb Haemost 1999; 81: 131-8.
26 Vidal C, Spaulding C, Picard F. et al. Flow cytometry detection of platelet procoagulation activity and microparticles in patients with unstable angina treated by percutaneous coronary angioplasty and stent implantation. Thromb Haemost 2001; 86: 784-90.
27 Aoki I, Shimoyama K, Aoki N. et al. Plateletdependent thrombin generation in patients with diabetes mellitus: effects of glycemic control on coagulability in diabetes. J Am Coll Cardiol 1996; 27: 560-6.
28 van der Planken MG, Vertessen FJ, Vertommen J. et al. Platelet prothrombinase activity, a final pathway platelet procoagulant activity, is overexpressed in type 1 diabetes: no relationship with mean platelet volume or background retinopathy. Clin Appl Thromb Hemost 2000; 06: 65-8.
29 Nakano M, Furutani M, Shinno H. et al. Elevation of soluble thrombomodulin antigen levels in the serum and urine of streptozotocin-induced diabetes model rats. Thromb Res 2000; 99: 83-91.
30 Ouvina SM, La Greca RD, Zanaro NL. et al. Endothelial dysfunction, nitric oxide and platelet activation in hypertensive and diabetic type II patients. Thromb Res 2001; 102: 107-14.
31 Min C, Kang E, Yu SH. et al. Advanced glycation end products induce apoptosis and procoagulant activity in cultured human umbilical vein endothelial cells. Diabetes Res Clin Pract 1999; 46: 197-202.
32 Matsunaga T, Iguchi K, Nakajima T. et al. Glycated high-density lipoprotein induces apoptosis of endothelial cells via a mitochondrial dysfunction. Biochem Biophys Res Commun 2001; 287: 714-20.
33 Claise C, Edeas M, Chaouchi N. et al. Oxidized-LDL induce apoptosis in HUVEC but not in the endothelial cell line EA.hy 926. Atherosclerosis 1999; 147: 95-104.
34 Boulanger CM, Scoazec A, Ebrahimian T. et al. Circulating microparticles from patients with myocardial infarction cause endothelial dysfunction. Circulation 2001; 104: 2649-52.
35 Barry OP, Pratico D, Lawson JA, FitzGerald GA. Transcellular activation of platelets and endothelial cells by bioactive lipids in platelet microparticles. J Clin Invest 1997; 99: 2118-27.
36 Nomura S, Tandon NN, Nakamura T. et al. High-shear-stress-induced activation of platelets and microparticles enhances expression of cell adhesion molecules in THP-1 and endothelial cells. Atherosclerosis 2001; 158: 277-87.
37 Huber J, Vales A, Mitulovic G. et al. Oxidized membrane vesicles and blebs from apoptotic cells contain biologically active oxidized phospholipids that induce monocyte-endothelial interactions. Arterioscler Thromb Vasc Biol 2002; 22: 101-7.
38 Nomura S, Imamura A, Okuno M. et al. Platelet-derived microparticles in patients with arteriosclerosis obliterans: enhancement of high shear-induced microparticle generation by cytokines. Thromb Res 2000; 98: 257-68.
39 Merten M, Pakala R, Thiagarajan P. et al. Platelet microparticles promote platelet interaction with subendothelial matrix in a glycoprotein IIb/IIIa-dependent mechanism. Circulation 1999; 99: 2577-82.
40 Nomura S, Shouzu A, Omoto S. et al. Effect of cilostazol on soluble adhesion molecules and platelet-derived microparticles in patients with diabetes. Thromb Haemost 1998; 80: 388-92.
41 Morel O, Jesel L, Hugel B. et al. Protective effects of Vitamin C on endothelium damage and platelet activation during myocardial infarction in patients with sustained generation of circulating microparticles. J Thromb Haemost 2003; 01: 171-7.
42 Blann AD, Lanza F, Galajda P. et al. Increased platelet glycoprotein V levels in patients with coronary and peripheral atherosclerosis—the influence of aspirin and cigarette smoking. Thromb Haemost 2001; 86: 777-83.
43 Sabatier V, Roux V, Anfosso F. et al. Interaction of endothelial microparticles with monocytic cells in vitro induces tissue factordependent procoagulant activity. Blood 2002; 99: 3962-70.
44 Mesri M, Altieri DC. Leukocyte microparticles stimulate endothelial cell cytokine release and tissue factor induction in a JNK1 signaling pathway. J Biol Chem 1999; 274: 23111-8.
45 Muller I, Klocke A, Alex M. et al. Intravascular tissue factors initiates coagulation via circulating microvesicles and platelets. FASEB J 2003; 17: 476-8.