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Thromb Haemost 2004; 92(01): 191-200
DOI: 10.1160/TH03-11-0687
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Antithrombotic effects of saratin on human atherosclerotic plaques

Gemma Vilahur
1   Cardiovascular Research Center, ICCC-CSIC, Hospital de la Santa Creu i Sant Pau (U.A.B), Barcelona, Spain
,
Xavier Duran
1   Cardiovascular Research Center, ICCC-CSIC, Hospital de la Santa Creu i Sant Pau (U.A.B), Barcelona, Spain
,
Oriol Juan-Babot
1   Cardiovascular Research Center, ICCC-CSIC, Hospital de la Santa Creu i Sant Pau (U.A.B), Barcelona, Spain
,
Laura Casaní
1   Cardiovascular Research Center, ICCC-CSIC, Hospital de la Santa Creu i Sant Pau (U.A.B), Barcelona, Spain
,
Lina Badimon
1   Cardiovascular Research Center, ICCC-CSIC, Hospital de la Santa Creu i Sant Pau (U.A.B), Barcelona, Spain
› Author AffiliationsFinancial support: This work has been possible thanks to the funds provided by Merck, KGaA, FIS-C0301 and PN-SAF 2000/0174.
Further Information

Publication History

Received 12 November 2003

Accepted after resubmission 04 May 2004

Publication Date:
29 November 2017 (online)

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Summary

Platelets play a primary role in thrombus formation after plaque rupture. Platelets recognize the exposed collagen via Von Willebrand factor (VWF) and become activated. Saratin, an inhibitor of the VWF-dependent binding of platelets to collagen, may reduce the thrombotic risk associated to atherosclerosis. Our objective was to evaluate the antithrombotic effects of local treatment with saratin on human atherosclerotic lesions. Thrombus formation was assessed by the deposition of 111Inplatelets on different human atherosclerotic lesions under three local shear conditions (800,1700 and 3400/s) with blood derived from catheterized pigs. Human atherosclerotic lesions were locally treated with saratin (30 µg/ml) at 37ºC for 5 min and placed in the chamber. Under stenotic shear conditions of 800/s, saratin significantly (p<0.05) reduced platelet deposition triggered by human denuded vessel wall (44%), fatty streaks (47%), severely damaged vessel (50%) and atherosclerotic plaque (57%). Thrombus characterization by immunohistochemistry showed also a reduction in fibrin deposition in treated vessels. These results suggest that the local site-specific treatment with saratin inhibits atherosclerotic plaque thrombogenicity at haemodynamic conditions typical of moderately stenotic coronary arteries.

Keywords

Von Willebrand Factor - collagen - saratin - platelets - thrombosis
 

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