Antibody cross-linking of human platelet P-selectin induces calcium entry by a mechanism dependent upon Fcγ receptor IIA

Jean G. Sathish; Shahrokh Falati; Kevin Croce; Colin Crump; Barbara C. Furie; Bruce Furie; Alastair W. Poole

doi:10.1160/TH03-11-0689

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 2004; 92(03): 598-605
DOI: 10.1160/TH03-11-0689

Platelets and Blood Cells

Schattauer GmbH

Antibody cross-linking of human platelet P-selectin induces calcium entry by a mechanism dependent upon Fcγ receptor IIA

Jean G. Sathish^*

¹Department of Pharmacology, University of Bristol, UK

,

Shahrokh Falati

¹Department of Pharmacology, University of Bristol, UK

,

Kevin Croce

²Center for Hemostasis and Thrombosis Research, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts, USA

,

Colin Crump^*

¹Department of Pharmacology, University of Bristol, UK

,

Barbara C. Furie

²Center for Hemostasis and Thrombosis Research, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts, USA

,

Bruce Furie

²Center for Hemostasis and Thrombosis Research, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts, USA

,

Alastair W. Poole

¹Department of Pharmacology, University of Bristol, UK

› Institutsangaben

Abstract

Summary

It is established that antibody-induced cross-linking of platelet surface receptors is able to activate platelets in a manner dependent upon FcγRIIA. This has not, however, previously been shown for the adhesion receptor P-selectin, and since there is evidence that P-selectin may couple to activation events, it was important to address whether antibody cross-linking of this receptor induced signalling events, and whether this was dependent on FcγRIIA. Here we show that although addition of soluble P-selectin ligand rPSGL-Ig alone is not able to induce calcium signalling, further addition of a full-length rabbit antihuman IgG leads to a sustained rise in [Ca²⁺]_i.This was due to an increase in the frequency and amplitude of transient calcium spiking in single platelets. The response was dependent upon engagement of both P-selectin and FcγRIIA since blocking antibodies to either receptor inhibited the response. The calcium rise is mediated primarily by induction of a calcium entry mechanism involving the Na⁺-Ca²⁺ exchanger operating in reverse mode, since it was blocked by inhibitors of Na⁺-Ca²⁺ exchange, bepridil and 5 mM NiCl₂.

Keywords

P-selectin - FcγRIIA - calcium - rPSGL-Ig - platelet

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Referenzen

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