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DOI: 10.1160/TH03-11-0703
Generation of a polyclonal rabbit anti-mouse tissue factor antibody by nucleic acid immunisation
Financial support: The Medical Research Council of the United Kingdom supported this work. ADM was supported by the British Heart FoundationPublication History
Received
19 November 2003
Accepted after revision
12 October 2004
Publication Date:
14 December 2017 (online)


Summary
Tissue factor (TF) the cellular receptor and cofactor for factor VII, initiates coagulation and has also been implicated in several coagulation-independent functions, including inflammation, angiogenesis and tumour metastasis. Investigations of TF expression in mouse models of these processes has been limited by the availability of antibodies that specifically recognise mouse TF.We have generated a rabbit polyclonal antibody to mTF by DNA immunisation. This has yielded an antiserum that recognises native mTF in immunohistochemical and flow cytometric analyses.Furthermore,the antiserum is inhibitory in coagulation assays.This antiserum will be a valuable investigative tool in the analysis of mTF expression.