Thromb Haemost 2005; 93(01): 160-164
DOI: 10.1160/TH03-11-0703
New Technologies and Diagnostic Tools
Schattauer GmbH

Generation of a polyclonal rabbit anti-mouse tissue factor antibody by nucleic acid immunisation

Andrew D. Mumford
1   Haemostasis and Thrombosis, MRC Clinical Sciences Centre, London, United Kingdom
,
Daxin Chen
2   Department of Immunology, The Faculty of Medicine, Imperial College, Hammersmith Hospital Campus, London, United Kingdom
,
Anthony Dorling
2   Department of Immunology, The Faculty of Medicine, Imperial College, Hammersmith Hospital Campus, London, United Kingdom
,
Geoffrey Kemball-Cook
1   Haemostasis and Thrombosis, MRC Clinical Sciences Centre, London, United Kingdom
,
John H. McVey
1   Haemostasis and Thrombosis, MRC Clinical Sciences Centre, London, United Kingdom
› Author Affiliations
Financial support: The Medical Research Council of the United Kingdom supported this work. ADM was supported by the British Heart Foundation
Further Information

Publication History

Received 19 November 2003

Accepted after revision 12 October 2004

Publication Date:
14 December 2017 (online)

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Summary

Tissue factor (TF) the cellular receptor and cofactor for factor VII, initiates coagulation and has also been implicated in several coagulation-independent functions, including inflammation, angiogenesis and tumour metastasis. Investigations of TF expression in mouse models of these processes has been limited by the availability of antibodies that specifically recognise mouse TF.We have generated a rabbit polyclonal antibody to mTF by DNA immunisation. This has yielded an antiserum that recognises native mTF in immunohistochemical and flow cytometric analyses.Furthermore,the antiserum is inhibitory in coagulation assays.This antiserum will be a valuable investigative tool in the analysis of mTF expression.