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DOI: 10.1160/TH04-12-0823
Local delivery of 17β-estradiol improves reendothelialization and decreases inflammation after coronary stenting in a porcine model
Financial support: Dr. Sirois is a recipient of a scholarship from the Canadian Institutes of Health Research. Dr. Tanguay is supported by the Fonds de la Recherche en Santé du Québec and the Heart and Stroke Foundation of Canada.Publikationsverlauf
Received: 20. Dezember 2004
Accepted after resubmission: 02. August 2005
Publikationsdatum:
14. Dezember 2017 (online)
![](https://www.thieme-connect.de/media/10.1055-s-00035024/200505/lookinside/thumbnails/10-1160-th04-12-0823-1.jpg)
Summary
In the current study, we investigated the effect of local intravascular delivery of 17β-estradiol (17β-E) on subsequent instent neointimal hyperplasia. Twenty-seven stents were implanted in coronary arteries of juvenile swine. Coronary arteries were randomized to local treatment with 17β-E or no drug therapy (control-vehicle treated). Twenty-eight days posttreatment, angiographic images revealed an improved minimal lumen diameter (2.2 ± 0.2 vs. 1.3 ± 0.2 mm, P < 0.005) and a reduction of late lumen loss (1.7 ± 0.2 vs. 2.3 ± 0.1 mm, P < 0.01) in 17β-E-treated vessels compared to control-vehicle treated. Histological analyses showed a reduction of stenosis (51.49 ± 6.75 vs.70.86 ± 6.24%, P < 0.05), mean neointimal thickness (0.51 ± 0.07 vs.0.83 ± 0.14 mm, P < 0.05) and inflammation score (1.29 ± 0.28 vs. 2.85 ± 0.40, P < 0.05) in 17β-E-treated arteries compared to control-vehicle treated arteries. Immunohistochemistry analyses revealed a reduction of proliferating smooth muscle cells and increased in-stent reendothelialization in 17β-E-treated arteries. Finally, we observed a correlation between neointimal hyperplasia and inflammation score, which in turn, was inversely related to reendothelialization. Locally delivered, 17β-E is inhibiting the inflammatory response and smooth muscle cells proliferation and improving vascular reendothelialization which together are contributing to reduce instent restenosis in a porcine coronary injury model. Together, these data demonstrate the potential clinical application of 17β-estradiol to improve vascular healing and prevent in-stent restenosis.
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