Understanding platelets

Marcus Dittrich; Ingvild Birschmann; Christiane Stuhlfelder; Albert Sickmann; Sabine Herterich; Bernhard Nieswandt; Ulrich Walter; Thomas Dandekar

doi:10.1160/TH05-02-0121

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2005; 94(05): 916-925
DOI: 10.1160/TH05-02-0121

Review Article

Schattauer GmbH

Understanding platelets

Lessons from proteomics, genomics and promises from network analysis

Marcus Dittrich

¹Department of Bioinformatics, Biocenter

²Department of Clinical Biochemistry and Pathobiochemistry, University of Würzburg, Würzburg, Germany

,

Ingvild Birschmann

²Department of Clinical Biochemistry and Pathobiochemistry, University of Würzburg, Würzburg, Germany

,

Christiane Stuhlfelder

³Rudolf Virchow-Center for Experimental Biomedicine, University of Würzburg, Würzburg, Germany

,

Albert Sickmann

³Rudolf Virchow-Center for Experimental Biomedicine, University of Würzburg, Würzburg, Germany

,

Sabine Herterich

²Department of Clinical Biochemistry and Pathobiochemistry, University of Würzburg, Würzburg, Germany

,

Bernhard Nieswandt

²Department of Clinical Biochemistry and Pathobiochemistry, University of Würzburg, Würzburg, Germany

³Rudolf Virchow-Center for Experimental Biomedicine, University of Würzburg, Würzburg, Germany

,

Ulrich Walter

²Department of Clinical Biochemistry and Pathobiochemistry, University of Würzburg, Würzburg, Germany

,

Thomas Dandekar

¹Department of Bioinformatics, Biocenter

⁴EMBL, Heidelberg, Germany

› Author Affiliations

Abstract

Summary

New large-scale analysis techniques such as bioinformatics, mass spectrometry and SAGE data analysis will allow a new framework for understanding platelets. This review analyses some important options and tasks for these tools and examines an outline of the new, refined picture of the platelet outlined by these new techniques. Looking at the platelet-specific building blocks of genome, (active) transcriptome and proteome (notably secretome and phospho-proteome), we summarize current bioinformatical and biochemical approaches, tasks as well as their limitations. Understanding the surprisingly complex platelet regarding compartmentalization, key cascades, and pathways including clinical implications will remain an exciting and hopefully fruitful challenge for the future.

Keywords

Pathway - mass spectrometry - transcriptome - platelet - post-transcriptional - coagulation - hemostasis

Full Text

References

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