Exercise paradoxically modulates oxidized low density lipoproteininduced adhesion molecules expression and trans-endothelial migration of monocyte in men

Jong-Shyan Wang; Yu-Wen Chen; Shu-Er Chow; Hsiu-Chung Ou; Wayne Huey-Herng Sheu

doi:10.1160/TH05-02-0139

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2005; 94(04): 846-852
DOI: 10.1160/TH05-02-0139

Endothelium and Vascular Development

Schattauer GmbH

Exercise paradoxically modulates oxidized low density lipoproteininduced adhesion molecules expression and trans-endothelial migration of monocyte in men

Jong-Shyan Wang

¹Graduate Institute of Rehabilitation Science and Center for Gerontological Research, Chang Gung University, Tao-Yuan, Taiwan

,

Yu-Wen Chen

¹Graduate Institute of Rehabilitation Science and Center for Gerontological Research, Chang Gung University, Tao-Yuan, Taiwan

,

Shu-Er Chow

²Department of Physiology, Chang Gung University, Tao-Yuan, Taiwan

,

Hsiu-Chung Ou

³Division of Endocrinology and Metabolism, Department of Education and Medical Research, Taichung Veterans General Hospital, Taichung, Taiwan

,

Wayne Huey-Herng Sheu

³Division of Endocrinology and Metabolism, Department of Education and Medical Research, Taichung Veterans General Hospital, Taichung, Taiwan

› Author Affiliations

Abstract

Summary

Physical exercise can affect the risk of cardiovascular disease. Oxidized-low density lipoprotein (ox-LDL) promotes transendothelial migration (TEM) of monocyte, thereby accelerating the pathogenesis of atherosclerosis. This study investigated how exercise intensity affects monocyte/EC interactions under ox LDL-mediated condition. Light (LIE), moderate (MIE) and high (HIE) intensity exercise (i.e., 40%, 60%, and 80%V . O2max, respectively) on a bicycle ergometer in 18 sedentary healthy men were performed on three separate occasions. Before and immediately after exercise, ox-LDL-promoted expressions of monocyte adhesion molecules and TEM of monocyte, as well as oxidation of LDL and amounts of soluble adhesion molecules in plasma were measured. Analytical results showed that (1) ox-LDL furthered monocyte L-selectin shedding and Mac-1 expression, and an attendant increase in TEM of monocyte, while treating the monocyte with Mac-1 antibody inhibited the ox-LDL-promoted TEM of monocyte; (2) under ox-LDL-treated condition, MIE increased monocyte Mac-1 and LFA-1 expressions, enhancing the TEM of monocyte, whereas HIE downregulated monocyte Mac-1 expression, suppressing theTEM of monocyte; (3) LIE decreased basal LFA-1 expression as well as basal and ox-LDL-promoted TEM of monocyte; and (4) MIE and HIE, but not LIE, elevated plasma ox-LDL level, while there were no significant changes in sL-selectin, sE-selectin, sICAM-1, and sVCAM-1 following these exercises. Therefore, we conclude that monocyte activation and subsequent TEM promoted by ox-LDL are changed by short-term exercise in an intensity-dependent manner. These findings provide a new insight into the may aid the development of suitable exercise intensity enable people to prevent early atherogenesis.

Keywords

Exercise - monocyte - endothelium - adhesion molecules - transmigration

Full Text

References

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