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Thromb Haemost 2005; 94(02): 454-459
DOI: 10.1160/TH05-03-0206
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Coagulation factor XIII-A

A flow cytometric intracellular marker in the classification of acute myeloid leukemias
János Kappelmayer
1   Departments of Clinical Biochemistry and Molecular Pathology, University of Debrecen, Debrecen, Hungary
,
Ágnes Simon
1   Departments of Clinical Biochemistry and Molecular Pathology, University of Debrecen, Debrecen, Hungary
,
Éva Katona
2   Departments of Clinical Research Center, University of Debrecen, Debrecen, Hungary
,
Attila Szanto
3   Department of Biochemistry and Molecular Biology, University of Debrecen, Debrecen, Hungary
,
László Nagy
3   Department of Biochemistry and Molecular Biology, University of Debrecen, Debrecen, Hungary
,
Attila Kiss
4   2nd Department of Medicine, University of Debrecen, Debrecen, Hungary
,
Csongor Kiss
5   Department of Pediatrics, Medical and Health Science Centre, University of Debrecen, Debrecen, Hungary
,
László Muszbek
1   Departments of Clinical Biochemistry and Molecular Pathology, University of Debrecen, Debrecen, Hungary
2   Departments of Clinical Research Center, University of Debrecen, Debrecen, Hungary
› Author AffiliationsFinancial support: The study was supported by the following OTKA grants: TS 044796, 043086, T 038307 and T 049392.
Further Information

Publication History

Received: 25 March 2005

Accepted after major revision: 04 June 2005

Publication Date:
05 December 2017 (online)

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Summary

The association of coagulation factors with leukocytes have been demonstrated in several previous studies. This study was designed to study the sensitivity and specificity of factor XIII subunit A (FXIII-A) labelling in cultured myeloblastic and monoblastic cell lines and to investigate the intracytoplasmic expression of FXIII-A in de novo acute myeloid leukemia (AML) samples. Myeloblastic and a monoblastic cell lines were cultured and investigated for lineage specific maturation markers and FXIII-A expression. Furthermore, FXIII-A expression was investigated in 12 normal samples (7 bone marrow and 5 peripheral blood), 86 de novo AML samples and 6 chronic myelomonocytic leukemia (CMML) samples. In the monoblastic MonoMac6 cell line the appearance of FXIII-A preceded that of CD14 while it remained negative in the myeloblastic PLB-985 cell line throughout its maturation period. Among the AML samples the average frequency of FXIII-A positive cells in myeloblastic leukemia samples was below 10%,while in M4 and M5AML samples it was above 50% and was significantly higher than the generally used CD14 marker (p < 0.0001). In the AML M4 and M5 cases, FXIII-A proved sensitive for the identification of monoblasts.FXIII-A can be considered as a reliable intracytoplasmic marker for the monocytic and megakaryocytic series and its presence is highly predictive for mono- and megakaryocytic AML and for CMML

Keywords

Factor XIII - flow cytometry - acute myeloid leukemia classification
 

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