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Thromb Haemost 2006; 95(03): 524-534
DOI: 10.1160/TH05-06-0457
Cellular Proteolysis and Oncology
Schattauer GmbH

Direct interaction of the kringle domain of urokinase-type plasminogen activator (uPA) and integrin αvβ3 induces signal transduction and enhances plasminogen activation

Takehiko Tarui
2   Department of Cell Biology, The Scripps Research Institute, La Jolla, California
,
Nobuaki Akakura
1   Department of Dermatology, University of California Davis Medical Center, Sacramento, California
,
Mousumi Majumdar
2   Department of Cell Biology, The Scripps Research Institute, La Jolla, California
,
Nicholas Andronicos
2   Department of Cell Biology, The Scripps Research Institute, La Jolla, California
,
Junichi Takagi
3   Center for Blood Research and Department of Pathology, Harvard Medical School, Boston, Massachussetts
,
Andrew P. Mazar
4   Attenuon, LLC, San Diego, California
,
Khalil Bdeir
5   Departments of Pathology and Laboratory Medicine, University of Pennsylvania, Pennsylvania, USA
,
Alice Kuo
5   Departments of Pathology and Laboratory Medicine, University of Pennsylvania, Pennsylvania, USA
,
Serge V. Yarovoi
5   Departments of Pathology and Laboratory Medicine, University of Pennsylvania, Pennsylvania, USA
,
Douglas B. Cines
5   Departments of Pathology and Laboratory Medicine, University of Pennsylvania, Pennsylvania, USA
,
Yoshikazu Takada
1   Department of Dermatology, University of California Davis Medical Center, Sacramento, California
› Author AffiliationsFinancial support: This paper was supported by National Institute of Health grants GM47157 (to YT) and HL60169 (to DBC).
Further Information

Publication History

Received 29 June 2005

Accepted after resubmission 03 January 2006

Publication Date:
29 November 2017 (online)

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Summary

It has been questioned whether there are receptors for urokinase-type plasminogen activator (uPA) that facilitate plasminogen activation other than the high affinity uPA receptor (uPAR/CD87) since studies of uPAR knockout mice did not support a major role of uPAR in plasminogen activation. uPA also promotes cell adhesion, chemotaxis, and proliferation besides plasminogen activation. These uPA-induced signaling events are not mediated by uPAR,but mediated by unidentified,lower-affinity receptors for the uPA kringle.We found that uPA binds specifically to integrin αvβ3 on CHO cells depleted of uPAR. The binding of uPA to αvβ3 required the uPA kringle domain. The isolated uPA kringle domain binds specifically to purified,recombinant soluble, and cell surface αvβ3, and other integrins (α4β1 and α9β1), and induced migration of CHO cells in an αvβ3-dependent manner. The binding of the uPA kringle to αvβ3 and uPA kringle-induced αvβ3-dependent cell migration were blocked by homologous plasminogen kringles 1-3 or 1-4 (angiostatin),a known integrin antagonist. We studied whether the binding of uPA to integrin αvβ3 through the kringle domain plays a role in plasminogen activation. On CHO cell depleted of uPAR, uPA enhanced plasminogen activation in a kringle and αvβ3-dependent manner.Endothelial cells bound to and migrated on uPA and uPA kringle in an αvβ3-dependent manner. These results suggest that uPA binding to integrins through the kringle domain plays an important role in both plasminogen activation and uPA-induced intracellular signaling. The uPA kringle-integrin interaction may represent a novel therapeutic target for cancer, inflammation, and vascular remodeling.

Keywords

Adhesion receptors / integrins - adhesion molecules - cell migration - angiogenesis and inhibitors - plasminogen activators
 

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