Association of factor V gene polymorphisms (Leiden; Cambridge; Hong Kong and HR2 haplotype) with recurrent idiopathic pregnancy loss in Tunisia

Walid Zammiti; Nabil Mtiraoui; Eric Mercier; Nesrine Abboud; Sarra Saidi; Touhami Mahjoub; Wassim Y. Almawi; Jean-Christophe Gris

doi:10.1160/TH05-07-0525

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2006; 95(04): 612-617
DOI: 10.1160/TH05-07-0525

Blood Coagulation, Fibrinolysis and Cellular Haemostasis

Schattauer GmbH

Association of factor V gene polymorphisms (Leiden; Cambridge; Hong Kong and HR2 haplotype) with recurrent idiopathic pregnancy loss in Tunisia

A case-control study

Walid Zammiti

¹Research Unit of Haematological and Autoimmune Diseases, Faculty of Pharmacy, Monastir, Center University, Tunisia

,

Nabil Mtiraoui

¹Research Unit of Haematological and Autoimmune Diseases, Faculty of Pharmacy, Monastir, Center University, Tunisia

,

Eric Mercier

²Laboratory of Haematology, Faculty of Biological and Pharmaceutical Sciences, Montpellier-1 University, Montpellier, France

,

Nesrine Abboud

¹Research Unit of Haematological and Autoimmune Diseases, Faculty of Pharmacy, Monastir, Center University, Tunisia

,

Sarra Saidi

¹Research Unit of Haematological and Autoimmune Diseases, Faculty of Pharmacy, Monastir, Center University, Tunisia

,

Touhami Mahjoub

¹Research Unit of Haematological and Autoimmune Diseases, Faculty of Pharmacy, Monastir, Center University, Tunisia

,

Wassim Y. Almawi

³Al-Jawhara Center for Molecular Medicine, Genetics and Inherited Diseases, College of Medicine and Medical Sciences, Arabian Gulf University, Manama, Bahrain

,

Jean-Christophe Gris

²Laboratory of Haematology, Faculty of Biological and Pharmaceutical Sciences, Montpellier-1 University, Montpellier, France

› Author Affiliations

Abstract

Summary

Inherited thrombophilia has been shown to be linked with fetal loss. We performed a case-control study on the association between thrombosis-related polymorphisms in the factor V (FV) gene (Leiden, Cambridge, Hong Kong; HR2 haplotype) and idiopathic recurrent pregnancy loss (RPL) in Tunisian women. A total of 348 women with RPL, and 203 control women were studied, corresponding to 1,250 pregnancy losses and 1,200 successful pregnancies. FV Leiden was seen in 19.4% of patients (4.3% in the homozygous state) and in 5.5% of controls. The prevalence of the FV HR2 haplotype was similar in patients and controls, but with 7 homozygous patients for 1 control. FV Cambridge and Hong Kong were absent from both patients and controls. The study of all pregnancy losses evidenced that the frequency of the factor V Leiden polymorphism was zero in women who had miscarried before7 weeks of gestation, and then sharply increased to a plateau. After categorization of pregnancy losses (before8 weeks of gestation; weeks 8 and 9; weeks 10 to 12; from the 13^th week of gestation onwards), heterozygous and homozygous factor V Leiden polymorphisms, and homozygous FV HR2 haplotype, were associated with significant and independent risks of pregnancy loss during weeks 8 and 9, which increased during weeks 10 to 12, then culminated after week 12. In Tunisian women with idiopathic RPL, factor V Leiden polymorphism and homozygous FV HR2 haplotype are not a risk factor for very early pregnancy loss, before 8 weeks of gestation, but are thereafter associated with significant clinical risks, which gradually increase from the 8^th week onwards.

Keywords

Pregnancy loss - Factor V Leiden mutation - Factor V HR2 haplotype

Full Text

References

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