The effect of glycaemic control on fibrin network structure of type 2 diabetic subjects

Marlien Pieters; Namukolo Covic; Du Toit Loots; Francois H. van der Westhuizen; Danie G. van Zyl; Paul Rheeder; Johann C. Jerling; John W. Weisel

doi:10.1160/TH06-07-0390

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2006; 96(05): 623-629
DOI: 10.1160/TH06-07-0390

Blood Coagulation, Fibrinolysis and Cellular Haemostasis

Schattauer GmbH

The effect of glycaemic control on fibrin network structure of type 2 diabetic subjects

Marlien Pieters

¹Department of Nutrition, North-West University, Potchefstroom, South Africa

,

Namukolo Covic

¹Department of Nutrition, North-West University, Potchefstroom, South Africa

,

Du Toit Loots

¹Department of Nutrition, North-West University, Potchefstroom, South Africa

,

Francois H. van der Westhuizen

²School of Biochemistry, North-West University, Potchefstroom, South Africa

,

Danie G. van Zyl

³Department of Internal Medicine, University of Pretoria, South Africa

,

Paul Rheeder

⁴Division of Clinical Epidemiology, University of Pretoria, Pretoria, South Africa

,

Johann C. Jerling

¹Department of Nutrition, North-West University, Potchefstroom, South Africa

,

John W. Weisel

⁵Department of Cell and Developmental Biology, University of Pennsylvania, Philadelphia, Pennsylvania, USA

› Author Affiliations

Abstract

Summary

Diabetic subjects have been shown to have altered fibrin network structures. One possible cause may be fibrinogen glycation resulting in altered structure/function properties. We investigated the effect of glucose control on fibrinogen glycation and fibrin network structure in type 2 diabetes. Blood samples were taken from twenty uncontrolled diabetic subjects at baseline to determine the levels of fibrinogen glycation and fibrin network structures. The subjects were then treated with insulin until blood glucose control was achieved before end blood samples were taken. Twenty age- and BMI-matched non-diabetic subjects were included as a reference group. The diabetic subjects had significantly higher mean fibrinogen glycation at baseline than the non-diabetic subjects (7.84 vs. 3.89 mol glucose / mol fibrinogen;p < 0.001).This was significantly reduced during the intervention (7.84 to 5.24 mol glucose / mol fibrinogen; p< 0.0002) in the diabetic group. Both groups had high mean fibrinogen concentrations (4.25 and 4.02 g/l, diabetic and non-diabetic subjects respectively). There was no difference in fibrinogen concentration, porosity, compaction and kinetics of clot formation between the diabetic subjects and non-diabetic subjects at baseline, nor were there any changes during the intervention despite the reduced fibrinogen glycation. Fibrin network characteristics correlated well with fibrinogen but not with any markers of glycaemic control. Improved glycaemic control resulted in decreased fibrinogen glycation but not fibrinogen concentration. It seems as though porosity, compaction and kinetics of clot formation are more related to fibrinogen concentration than fibrinogen glycation in this model.

Keywords

Diabetes - glycation - fibrinogen - fibrin network structure - glucose control

Full Text

References

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