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Thromb Haemost 2007; 98(04): 896-899
DOI: 10.1160/TH07-03-0188
Case Report
Schattauer GmbH

Impaired platelet responsiveness to clopidogrel identified by flow cytometric vasodilator-stimulated phosphoprotein (VASP) phosphorylation in patients with subacute stent thrombosis

Olivier Morel
1   Fédération de Cardiologie des Hôpitaux Universitaires de Strasbourg, France
2   Institut d’hématologie et d’Immunologie, Faculté de Médecine, Strasbourg et Unité 770 INSERM, France
,
Antoine Faure
1   Fédération de Cardiologie des Hôpitaux Universitaires de Strasbourg, France
,
Patrick Ohlmann
1   Fédération de Cardiologie des Hôpitaux Universitaires de Strasbourg, France
,
Laurence Jesel
1   Fédération de Cardiologie des Hôpitaux Universitaires de Strasbourg, France
,
Dominique Desprez
3   Unité d’Hémostase, CH Strasbourg, France
,
Lelia Grunebaum
3   Unité d’Hémostase, CH Strasbourg, France
,
Pierre Bareiss
1   Fédération de Cardiologie des Hôpitaux Universitaires de Strasbourg, France
› Author Affiliations
Further Information

Publication History

Received 13 March 2007

Accepted after resubmission 20 July 2007

Publication Date:
01 December 2017 (online)

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  • References

  • 1 Cattaneo M. Aspirin and clopidogrel: efficacy, safety, and the issue of drug resistance. Arterioscler Thromb Vasc Biol 2004; 24: 1980-1987.
    CrossrefPubMedGoogle Scholar
  • 2 Ajzenberg N, Aubry P, Huisse MG. et al. Enhanced shear-induced platelet aggregation in patients who experience subacute stent thrombosis: a case-control study. J Am Coll Cardiol 2005; 45: 1753-1756.
    CrossrefPubMedGoogle Scholar
  • 3 Barragan P, Bouvier JL, Roquebert PO. et al. Resistance to thienopyridines: clinical detection of coronary stent thrombosis by monitoring of vasodilator-stimulated phosphoprotein phosphorylation. Catheter Cardiovasc Interv 2003; 59: 295-302.
    CrossrefPubMedGoogle Scholar
  • 4 Wenaweser P, Dorffler-Melly J, Imboden K. et al. Stent thrombosis is associated with an impaired response to antiplatelet therapy. J Am Coll Cardiol 2005; 45: 1748-1752.
    CrossrefPubMedGoogle Scholar
  • 5 Schafer A, Bonz AW, Eigenthaler M. et al. Late thrombosis of a drug-eluting stent during combined anti-platelet therapy in a clopidogrel nonresponsive diabetic patient: Shall we routinely test platelet function?. Thromb Haemost 2007; 97: 862-865.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 6 von Beckerath N, Taubert D, Pogatsa-Murray G. et al. A patient with stent thrombosis, clopidogrel-resistance and failure to metabolize clopidogrel to its active metabolite. Thromb Haemost 2005; 93: 789-791.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 7 Biondi-Zoccai GGE, Sangiogri GM, Chieffo A. et al. Validation of predictors of intraprocedural stent thrombosis in the drug-eluting stent era. Am J Cardiol 2005; 95: 1466-1468.
    CrossrefPubMedGoogle Scholar
  • 8 Kuchulakanti PK, Chu WW, Torguson R. et al. Correlates and long term outcomes of angiographically proven stent thrombosis with sirolimus and paclitaxeleluting stents. Circulation 2006; 111: 1108-1113.
    PubMedGoogle Scholar
  • 9 Smit JJ, van’t Hof AW, de Boer MJ. et al. Incidence and predictors of subacute thrombosis in patients undergoing primary angioplasty for an acute myocardial infarction. Thromb Haemost 2006; 96: 190-195.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 10 Angiolillo DJ, Fernandez-Ortiz A, Bernardo E. et al. Platelet aggregation according to body mass index in patients undergoing coronary stenting: should clopidogrel loading-dose be weight adjusted?. J Invasive Cardiol 2004; 16: 169-174.
    PubMedGoogle Scholar
  • 11 Grossmann R, Sokolova O, Schnurr A. et al. Variable extent of clopidogrel responsiveness in patients after coronary stenting. Thromb Haemost 2004; 92: 1201-1206.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 12 Aleil B, Meyer N, Cazenave JP. et al. High stability of blood samples for flow cytometric analysis of VASP phosphorylation to measure the clopidogrel responsiveness in patients with coronary artery disease. Thromb Haemost 2005; 94: 886-887.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 13 Gurbel PA, Bliden KP, Hiatt BL. et al. Clopidogrel for coronary stenting: response variability, drug resistance, and the effect of pretreatment platelet reactivity. Circulation 2003; 107: 2908-2913.
    CrossrefPubMedGoogle Scholar
  • 14 Serebruany VL, Steinhubl SR, Berger PB. et al. Variability in platelet responsiveness to clopidogrel among 544 individuals. J Am Coll Cardiol 2005; 45: 246-251.
    CrossrefPubMedGoogle Scholar
  • 15 Matetzky S, Shenkman B, Guetta V. et al. Clopidogrel resistance is associated with increased risk of recurrent atherothrombotic events in patients with acute myocardial infarction. Circulation 2004; 109: 3171-3175.
    CrossrefPubMedGoogle Scholar
  • 16 Cuisset T, Frere C, Quilici J. et al. High post-tratment platelet reactivity identified low-responders to dual antiplatelet therapy at increased risk of recurrent cardiovascular events after stenting for acute coronary syndrome. J Thromb Haemost 2006; 3: 542-549.
    PubMedGoogle Scholar
  • 17 Hochholzer W, Trenk D, Bestehorn HP. et al. Impact of the degree of peri-interventional platelet inhibition after loading with clopidogrel on early clinical outcome of elective coronary stent placement. J Am Coll Cardiol 2006; 48: 1742-1750.
    CrossrefPubMedGoogle Scholar
  • 18 Gurbel PA, Bliden KP, Samara WM. et al. Clopidogrel effect on platelet reactivity in patients with stent thrombosis. Results of the CREST study. J Am Coll Cardiol 2005; 46: 1827-1832.
    CrossrefPubMedGoogle Scholar
  • 19 Obergfell A, Strotmann J, Bonz A. et al. Impaired platelet responses to clopidogrel and ticlopidine in a patient with recurrent coronary stent stenosis. Thromb Haemost 2004; 92: 1446-1447.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 20 Buonamici P, Marucci R, Migliorini A. et al. Impact of platelet reactivity after clopidogrel administration on drug-eluting stent thrombosis. J Am Coll Cardiol 2007; 49: 2312-2317.
    CrossrefPubMedGoogle Scholar
  • 21 Labarthe B, Théroux P, Angioï M. et al. Matching the evaluation of the clinical efficacy of clopidogrel to platelet function tests relevant to the biological properties of the drug. J Am Coll Cardiol 2005; 46: 638-645.
    CrossrefPubMedGoogle Scholar
  • 22 Malinin A, Pokov A, Spergling M. et al. Monitoring Platelet inhibition after clopidogrel with the verifyNow- P2Y12 rapide analyser: the verify thrombosis risk assessment (VERITAS) study. Thromb Res 2006; 119: 277-284.
    PubMedGoogle Scholar
  • 23 Gurbel PA, Tantry US. Aspirin and clopidogrel resistance: Consideration and management. J Interv Cardiol 2006; 19: 439-448.
    CrossrefPubMedGoogle Scholar
  • 24 Siller-Matula J, Schror K, Wojta J. et al. Thienopyridines in cardiovascular disease: focus on clopidogrel resistance. Thromb Haemost 2007; 97: 385-393.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 25 Toth O, Calatzis A, Penz S. et al. Multiple electrode aggregometry: a new device to measure platelet aggregation in whole blood. Thromb Haemost 2006; 96: 781-788.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 26 Schwarz UR, Geiger J, Walter U. et al. Flow cytometry analysis of intracellular VASP phosphorylation for the assessment of activating and inhibitory signal transduction pathways in human platelets--definition and detection of ticlopidine/clopidogrel effects. Thromb Haemost 1999; 82: 1145-1152.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 27 Aleil B, Ravanat C, Cazenave JP. et al. Flow cytometric analysis of intraplatelet VASP phosphorylation for the detection of clopidogrel resistance in patients with ischemic cardiovascular diseases. J Thromb Haemost 2005; 3: 85-92.
    CrossrefPubMedGoogle Scholar
  • 28 Pampuch A, Cerletti C, de Gaetano G. Comparison of VASP-phosphorylation assay to light-transmission aggregometry in assessing inhibition of the platelet ADP P2Y12 receptor. Thromb Haemost 2006; 96: 767-773.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 29 Iakovou I, Schmidt T, Bonizzoni E. et al. Incidence, predictors, and outcome of thrombosis after successful implantation of drug-eluting stents. J Am Med Assoc 2005; 17: 2126-2130.
    PubMedGoogle Scholar
  • 30 Hacquard M, Angioi M, Lecompte T. et al. Interindividual variability in the laboratory response to the aspirin-clopidogrel combination: are patients with stent occlusion ‘resistant’ to treatment?. Ann Pharm Fr 2007; 65: 169-173.
    CrossrefPubMedGoogle Scholar