Effects of von Willebrand factor concentration and platelet collision on shear-induced platelet activation

Zhenyue Gao; Fang Liu; Ziqiang Yu; Xia Bai; Chun Yang; Fengyuan Zhuang; Changgeng Ruan

doi:10.1160/TH07-03-0222

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2008; 100(01): 60-68
DOI: 10.1160/TH07-03-0222

Platelets and Blood Cells

Schattauer GmbH

Effects of von Willebrand factor concentration and platelet collision on shear-induced platelet activation

Zhenyue Gao

¹Bioengineering Department of Beijing University of Aeronautics and Astronautics, Beijing, China

,

Fang Liu

²Jiangsu Institute of Hematology, Suzhou, China

,

Ziqiang Yu

²Jiangsu Institute of Hematology, Suzhou, China

,

Xia Bai

²Jiangsu Institute of Hematology, Suzhou, China

,

Chun Yang

¹Bioengineering Department of Beijing University of Aeronautics and Astronautics, Beijing, China

,

Fengyuan Zhuang

¹Bioengineering Department of Beijing University of Aeronautics and Astronautics, Beijing, China

,

Changgeng Ruan

²Jiangsu Institute of Hematology, Suzhou, China

› Author Affiliations

Abstract

Summary

The binding of plasma von Willebrand factor (vWF) to platelet glycoprotein (GP) Ibα in a high shear stress field, and subsequent integrin-GPIIb/IIIa-vWF conjunction induces platelet aggregation (SIPA). However, the specific biomechanical mechanism of the vWF-GPIb interaction still remains to be elucidated. A parallel-plate rectangular flow chamber was built to simulate a stenopeic artery flow pattern. Using the flow chamber, we examined shear- induced platelet activation (SIPAct) at different vWF concentrations (5–25 µg/ml) and several simulated stenotic high shear rates. P-selectin expression on the platelets and annexin V binding to the platelets were used as two markers of platelet activation. At different localized shear rates (3,000 s^-1–9,500 s^-1), the percentage of annexin V and P-selectin positive cells increased from 8.3 ± 0.4% to 22.3 ± 1.8% ( p 0.05) and from 17.4 ± 0.5% to 33.5 ± 2.5% (p 0.05),respectively. As the vWF concentration increased from 5 µg/ml to 25 µg/ml, the annexinV binding rate increased from 7.2 ± 0.6% to 53.4 ± 3.8% (p 0.05), and P-selectin expression increased from 16.5 ± 1.2% to 65.9 ± 5.2% (p 0.05). A test in a uniform shear field using cone-plate viscometer rheometry showed that the platelet activation rate was proportional to the platelet concentration. This result suggests that platelet collision is one of the impact factors of SIPAct.

Keywords

vWF - SIPA(ct) - flow chamber - cone-plate viscometer - platelet collision

Full Text

References

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