Factor XIa and tissue factor activity in patients with coronary artery disease

Saulius Butenas; Anetta Undas; Matthew T Gissel; Konstanty Szuldrzynski; Krzysztof Zmudka; Kenneth G Mann

doi:10.1160/TH07-08-0499

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2008; 99(01): 142-149
DOI: 10.1160/TH07-08-0499

Cardiovascular Biology and Cell Signalling

Schattauer GmbH

Factor XIa and tissue factor activity in patients with coronary artery disease

Saulius Butenas

¹University of Vermont, Department of Biochemistry, Burlington, Vermont, USA

,

Anetta Undas

²Institute of Cardiology

,

Matthew T Gissel

¹University of Vermont, Department of Biochemistry, Burlington, Vermont, USA

,

Konstanty Szuldrzynski

³Department of Medicine, Jagiellonian University School of Medicine, Krakow, Poland

,

Krzysztof Zmudka

²Institute of Cardiology

,

Kenneth G Mann

¹University of Vermont, Department of Biochemistry, Burlington, Vermont, USA

› Author Affiliations

Abstract

Summary

It has been established that inflammation and enhanced procoagulant activity are associated with the pathogenesis of atherosclerotic vascular disease. We evaluated and compared the contributions of the factor (F)XIa and tissue factor (TF) activity in plasma of patients with coronary artery disease (CAD). Citrate plasma was obtained prior to therapy from 53 patients with stable angina (29 with a history of previous myocardial infarction; CAD-MI) and 30 with acute coronary syndrome (ACS) within 12 hours from pain onset. Four ACS patients treated with heparin were excluded. FXIa andTF activity were determined in clotting assays based upon the prolongation of clotting time by inhibitory monoclonal antibodies. Twenty-five of 26ACS patients (96%) and 22 of 29 CAD-MI patients (76%) had quantifiable FXIa (50 ± 33 and 42 ± 45pM, respectively).Ten of 26 (38%) ACS patients and only three of 53 (6%) stable CAD patients showedTF activity (<0.4pM). No FXIa or TF activity was observed in agematched healthy controls (n=12).For both CAD-MI andACS patients, there were correlations (p<0.05) between FXIa and interleukin-6 (R²= 0.59 and 0.39, respectively) and between FXIa and TAT (R²= 0.64 and 0.63, respectively). In conclusion, the majority of ACS and CAD-MI patients have circulating FXIa that correlates with markers of coagulation and inflammation.

Keywords

Coronary artery disease - factor XIa - tissue factor - inflammation markers - coagulation markers

Full Text

References

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