Successful in vivo propagation of factor IX-producing hepatocytes in mice: Potential for cell-based therapy in haemophilia B

Kohei Tatsumi; Kazuo Ohashi; Miho Kataoka; Chise Tateno; Masaru Shibata; Hiroyuki Naka; Midori Shima; Michiyoshi Hisanaga; Hiromichi Kanehiro; Teruo Okano; Katsutoshi Yoshizato; Yoshiyuki Nakajima; Akira Yoshioka

doi:10.1160/TH07-09-0559

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2008; 99(05): 883-891
DOI: 10.1160/TH07-09-0559

Blood Coagulation, Fibrinolysis and Cellular Haemostasis

Schattauer GmbH

Successful in vivo propagation of factor IX-producing hepatocytes in mice: Potential for cell-based therapy in haemophilia B

Kohei Tatsumi

¹Department of Pediatrics, Nara Medical University, Nara, Japan

,

Kazuo Ohashi

²Department of Surgery, Nara Medical University, Nara, Japan

⁵Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo, Japan

,

Miho Kataoka

³Yoshizato Project, Cooperative Link of Unique Science and Technology for Economy Revitalization (CLUSTER), Hiroshima Prefectural Institute of Industrial Science and Technology, Hiroshima, Japan

,

Chise Tateno^#

³Yoshizato Project, Cooperative Link of Unique Science and Technology for Economy Revitalization (CLUSTER), Hiroshima Prefectural Institute of Industrial Science and Technology, Hiroshima, Japan

,

Masaru Shibata

¹Department of Pediatrics, Nara Medical University, Nara, Japan

,

Hiroyuki Naka

¹Department of Pediatrics, Nara Medical University, Nara, Japan

,

Midori Shima

¹Department of Pediatrics, Nara Medical University, Nara, Japan

,

Michiyoshi Hisanaga

²Department of Surgery, Nara Medical University, Nara, Japan

,

Hiromichi Kanehiro

²Department of Surgery, Nara Medical University, Nara, Japan

,

Teruo Okano

⁵Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo, Japan

,

Katsutoshi Yoshizato^#

³Yoshizato Project, Cooperative Link of Unique Science and Technology for Economy Revitalization (CLUSTER), Hiroshima Prefectural Institute of Industrial Science and Technology, Hiroshima, Japan

⁴Graduate School of Science, Hiroshima University, Hiroshima, Japan

,

Yoshiyuki Nakajima

²Department of Surgery, Nara Medical University, Nara, Japan

,

Akira Yoshioka

¹Department of Pediatrics, Nara Medical University, Nara, Japan

› Author Affiliations

Abstract

Summary

Cell-based therapies using isolated hepatocytes have been proposed to be an attractive application in the treatment of haemophilia B due to the normal production of coagulation factor IX (FIX) in these particular cells. Current cell culture technologies have largely failed to provide adequate isolated hepatocytes, so the present studies were designed to examine a new approach to efficiently proliferate hepatocytes that can retain normal biological function, including the ability to synthesize coagulation factors like FIX. Canine or human primary hepatocytes were transplanted into urokinase-type plasminogen activatorsevere combined immunodeficiency (uPA/SCID) transgenic mice. Both donor hepatocytes from canines and humans were found to progressively proliferate in the recipient mouse livers as evidenced by a sharp increase in the circulating blood levels of species-specific albumin, which was correlated with the production and release of canine and human FIX antigen levels into the plasma. Histological examination confirmed that the transplanted canine and human hepatocytes were able to proliferate and occupy >80% of the host livers. In addition, the transplanted hepatocytes demonstrated strong cytoplasmic staining for human FIX, and the secreted coagulation factor IX was found to be haemostatically competent using specific procoagulant assays. In all, the results from the present study indicated that developments based on this technology could provide sufficient FIX-producing hepatocytes for cell-based therapy for haemophilia B.

Keywords

Haemophilia A/B - haemophilia therapy - coagulation factors - hepatology

Full Text

References

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