Increased platelet adhesion under flow conditions is induced by both thalassemic platelets and red blood cells

Neta Goldschmidt; Galia Spectre; Alexander Brill; Orly Zelig; Ada Goldfarb; Eliezer Rachmilewitz; David Varon

doi:10.1160/TH08-03-0157

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2008; 100(05): 864-870
DOI: 10.1160/TH08-03-0157

Platelets and Blood Cells

Schattauer GmbH

Increased platelet adhesion under flow conditions is induced by both thalassemic platelets and red blood cells

Neta Goldschmidt^*

¹Department of Haematology and Coagulation Unit, Hadassah Hebrew University Medical Center, Jerusalem, Israel

,

Galia Spectre^*

¹Department of Haematology and Coagulation Unit, Hadassah Hebrew University Medical Center, Jerusalem, Israel

,

Alexander Brill

¹Department of Haematology and Coagulation Unit, Hadassah Hebrew University Medical Center, Jerusalem, Israel

,

Orly Zelig

¹Department of Haematology and Coagulation Unit, Hadassah Hebrew University Medical Center, Jerusalem, Israel

,

Ada Goldfarb

¹Department of Haematology and Coagulation Unit, Hadassah Hebrew University Medical Center, Jerusalem, Israel

,

Eliezer Rachmilewitz

²Department of Haematology, Edit Wolfson Medical Center, Holon, Israel

,

David Varon

¹Department of Haematology and Coagulation Unit, Hadassah Hebrew University Medical Center, Jerusalem, Israel

› Author Affiliations

Abstract

Summary

Thromboembolic complications are not uncommon in thalassemia. Previous studies suggest increased platelet aggregation and a potential role of pathological changes in the red blood cell (RBC) lipid membrane, induced by oxidative stress. In the present study,platelet adhesion and the effect of thalassemic RBC on platelet adhesion under flow conditions were evaluated, using the Cone and Plate (let)Analyzer(CPA).Twenty-two β-thalasse-mia patients and 22 blood type-matched healthy controls were studied. An increased platelet adhesion (% surface coverage, SC),was observed in patients as compared to controls (p<0.05). When platelet count and haematocrit were normalized by auto-logous reconstitution, a significant increase in platelet aggregation (average size,AS) was observed (p<0.05). Increased platelet adhesion (SC and AS), was demonstrated in six patients with a history of thrombosis as compared to 16 patients without any history of thrombosis (p≤0.007) and in 17 splenectomized patients as compared to five non-splenectomized patients (p=0.003). In reconstitution studies, thalassemic RBC mixed with normal platelet-rich plasma significantly increased platelet adhesion compared to normal RBC (SC p<0.03, AS p<0.02). Thalassemic platelets reconstituted with normal RBC, had increased aggregation (AS, p<0.004) in comparison with normal platelets.The results indicate that increased platelet adhesion in β-thalassemia is induced by both platelets and RBC. Increased platelet adhesion correlated with clinical thrombotic events and thus may suggest a mechanism of thrombosis in thalassemic patients.The potential application of the CPA in identifying thalassemic patients with high risk for thrombosis should be studied prospectively in a larger cohort of patients.

Keywords

β-thalassemia - thrombosis - red blood cells - platelets - adhesion - aggregation

Full Text

References

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