Enzymatically hydrolyzed low-density lipoprotein modulates inflammatory responses in endothelial cells

Dominic Fenske; Katrin Dersch; Cornelia Lux; Lisa Zipse; Prapat Suriyaphol; Yolantha Dragneva; Shan-Rui Han; Sucharit Bhakdi; Matthias Husmann

doi:10.1160/TH08-03-0166

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 2008; 100(06): 1146-1154
DOI: 10.1160/TH08-03-0166

Endothelium and Vascular Development

Schattauer GmbH

Enzymatically hydrolyzed low-density lipoprotein modulates inflammatory responses in endothelial cells

Dominic Fenske

¹Institute of Medical Microbiology and Hygiene, Johannes Gutenberg-University Mainz, Mainz, Germany

,

Katrin Dersch

¹Institute of Medical Microbiology and Hygiene, Johannes Gutenberg-University Mainz, Mainz, Germany

,

Cornelia Lux

¹Institute of Medical Microbiology and Hygiene, Johannes Gutenberg-University Mainz, Mainz, Germany

,

Lisa Zipse

¹Institute of Medical Microbiology and Hygiene, Johannes Gutenberg-University Mainz, Mainz, Germany

,

Prapat Suriyaphol

¹Institute of Medical Microbiology and Hygiene, Johannes Gutenberg-University Mainz, Mainz, Germany

,

Yolantha Dragneva

¹Institute of Medical Microbiology and Hygiene, Johannes Gutenberg-University Mainz, Mainz, Germany

,

Shan-Rui Han

¹Institute of Medical Microbiology and Hygiene, Johannes Gutenberg-University Mainz, Mainz, Germany

,

Sucharit Bhakdi

¹Institute of Medical Microbiology and Hygiene, Johannes Gutenberg-University Mainz, Mainz, Germany

,

Matthias Husmann

¹Institute of Medical Microbiology and Hygiene, Johannes Gutenberg-University Mainz, Mainz, Germany

› Institutsangaben

Abstract

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Summary

There is evidence that low-density lipoprotein (LDL) is modified by hydrolytic enzymes,and that the product (E-LDL) induces selective production of interleukin 8 (IL-8) in endothelial cells. Since nuclear factor-kappaB (NF-κB) is a major regulator of IL-8 transcription, we studied its activation in endothelial cells treated with E-LDL. Unexpectedly,the modified lipoprotein not only failed to activate NF-κB, but completely blocked its activation by tumour necrosis factor-alpha (TNF-α) in EA.hy926-cells, as assessed by electrophoretic mobility shift assays and immunofluorescence. Inhibition occurred upstream of NF-κB translocation, as inhibitor of NF-κB- (IκB)-phosphorylation was suppressed by E-LDL. In contrast to NF-κB,transcription factor activator protein-1 (AP-1) proved to be activated. Removal of free fatty acids present in E-LDL obliterated both activation of AP-1 and inhibition of NF-κB. Chromatin immunoprecipitation revealed that phosphorylated c-jun, but not NF-κBp65 bound to the natural IL-8 promoter. Production of endothelial IL-8 and simultaneous modulation of NF-κB in response to hydrolyzed LDL might serve to protect the vessel wall and promote silent removal of the insudated lipoprotein.

Keywords

Enzymatically modified LDL - NF-κB - AP-1 - interleukin-8 - endothelial cells

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Referenzen

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