Influence of platelet count on the expression of platelet collagen receptor glycoprotein VI (GPVI) in patients with acute coronary syndrome

Boris Bigalke; Konstantinos Stellos; Dimitrios Stakos; Thomas Joos; Oliver Pötz; Tobias Geisler; Christian Bischofs; Elisabeth Kremmer; Björn F. Krämer; Peter Seizer; Andreas E. May; Stephan Lindemann; Meinrad Gawaz

doi:10.1160/TH08-06-0399

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2009; 101(05): 911-915
DOI: 10.1160/TH08-06-0399

Platelets and Blood Cells

Schattauer GmbH

Influence of platelet count on the expression of platelet collagen receptor glycoprotein VI (GPVI) in patients with acute coronary syndrome

Boris Bigalke

¹Medizinische Klinik, Klinik für Kardiologie und Kreislauferkrankungen, Eberhard-Karls-Universität Tübingen, Germany

,

Konstantinos Stellos

¹Medizinische Klinik, Klinik für Kardiologie und Kreislauferkrankungen, Eberhard-Karls-Universität Tübingen, Germany

,

Dimitrios Stakos

¹Medizinische Klinik, Klinik für Kardiologie und Kreislauferkrankungen, Eberhard-Karls-Universität Tübingen, Germany

,

Thomas Joos

²Biochemistry Department, NMI – Natural and Medical Sciences Institute at the University of Tübingen, Reutlingen, Germany

,

Oliver Pötz

²Biochemistry Department, NMI – Natural and Medical Sciences Institute at the University of Tübingen, Reutlingen, Germany

,

Tobias Geisler

¹Medizinische Klinik, Klinik für Kardiologie und Kreislauferkrankungen, Eberhard-Karls-Universität Tübingen, Germany

,

Christian Bischofs

¹Medizinische Klinik, Klinik für Kardiologie und Kreislauferkrankungen, Eberhard-Karls-Universität Tübingen, Germany

,

Elisabeth Kremmer

³Institute for Molecular Immunology, GSF-National Research Center for Environment and Health, Helmoltz Center, Munich, Germany

,

Björn F. Krämer

¹Medizinische Klinik, Klinik für Kardiologie und Kreislauferkrankungen, Eberhard-Karls-Universität Tübingen, Germany

,

Peter Seizer

¹Medizinische Klinik, Klinik für Kardiologie und Kreislauferkrankungen, Eberhard-Karls-Universität Tübingen, Germany

,

Andreas E. May

¹Medizinische Klinik, Klinik für Kardiologie und Kreislauferkrankungen, Eberhard-Karls-Universität Tübingen, Germany

,

Stephan Lindemann

¹Medizinische Klinik, Klinik für Kardiologie und Kreislauferkrankungen, Eberhard-Karls-Universität Tübingen, Germany

,

Meinrad Gawaz

¹Medizinische Klinik, Klinik für Kardiologie und Kreislauferkrankungen, Eberhard-Karls-Universität Tübingen, Germany

› Author Affiliations

Abstract

Summary

Platelets play a key role in the development of an acute coronary syndrome (ACS) and contribute to cardiovascular events. Platelet collagen receptor glycoprotein VI (GPVI) contributes significantly to platelet adhesion and thrombus formation in ACS. We consecutively investigated both the platelet count and the platelet surface expression of GPVI in 843 patients with a symptomatic coronary artery disease verified by coronary angiography. Four hundred fourteen patients presented with stable angina pectoris and 429 patients with ACS. Platelet surface expression of GPVI and CD62P was determined by flow cytometry and platelet count with a coulter counter, plasmatic soluble GPVI was measured by ELISA. Platelet GPVI expression in patients with ACS was compared to platelet count. Patients with ACS showed significantly elevated GPVI expression levels in the first and second quartiles of platelet count compared to patients with higher platelet count [mean fluorescence intensity (MFI) ± standard deviation): 1^st vs. 4^th: 20.44 ± 6.1 vs. 18.62 ± 3.7; p=0.012; 2^ndvs.3^rd:21.2±8.5vs.18.76±3.7;P=0.03; 2^ndvs.4^th: 21.2±8.5vs.18.62±3.7;P=0.004], which was paralleled in trend for the CD62P expression [MFI: 1^st vs. 4^th: 11.2 ± 6.8 vs. 12.3 ± 9; p=0.057; 2^nd vs. 3^rd: 16.3 ± 16 vs.12.7 ± 5.3; p=0.138; 2^nd vs. 4^th: 16.3 ± 16 vs.11 ± 4.4; p=0.043]. In a subgroup of 48 patients with ACS, determination of soluble GPVI showed similar results [plasma GPVI (ng/ml): 1^stvs.4^th: 1.6 ± 0.6 vs. 1.2 ± 0.4; p=0.046; 1^st vs. 3^rd: 1.6 ± 0.6 vs. 1.1 ± 0.5; p=0.038; 2^nd vs. 3^rd: 1.9 ± 0.8 vs. 1.1 ± 0.5; p=0.04; 2^nd vs. 4^th: 1.9 ± 0.8 vs. 1.2 ± 0.4; p=0.056]. Thus, a lower platelet count comes along with a higher GPVI surface expression and plasma concentration in patients with ACS, which potentially reflects increased activation and enhanced recruitment of platelets to the site of vascular injury.

Keywords

Platelet count - glycoprotein VI - acute coronary syndrome

Full Text

References

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