Coagulation factor XIII serves as protein disulfide isomerase

Judith Lahav; Eli Karniel; Zsuzsa Bagoly; Vera Sheptovitsky; Rima Dardik; Aida Inbal

doi:10.1160/TH08-09-0605

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2009; 101(05): 840-844
DOI: 10.1160/TH08-09-0605

Blood Coagulation, Fibrinolysis and Cellular Haemostasis

Schattauer GmbH

Coagulation factor XIII serves as protein disulfide isomerase

Judith Lahav^*

¹Hemostasis Laboratory, Beilinson Hospital, Rabin Medical Center, Petah Tikva, Israel

,

Eli Karniel^*

²Internal Medicine Department B, Meir Hospital, Kfar Saba, Israel

,

Zsuzsa Bagoly

³Clinical Research Center, Haemostasis, Thrombosis and Vascular Biology Research Group of the Hungarian Academy of Sciences, University of Debrecen, Medical & Health Science Center, Debrecen, Hungary

,

Vera Sheptovitsky

¹Hemostasis Laboratory, Beilinson Hospital, Rabin Medical Center, Petah Tikva, Israel

,

Rima Dardik

⁴Thrombosis and Hemostasis Unit, Sheba Medical Center, Tel Hashomer, Israel

,

Aida Inbal

⁵Thrombosis and Hemostasis Unit, Department of Hematology, Beilinson Hospital, Rabin Medical Center, Petah Tikva, and Sackler Faculty of Medicine, Tel Aviv University, Israel

› Author Affiliations

Abstract

Summary

Tissue transglutaminase was reported to act as protein disulfide isomerase (PDI). We studied whether plasma transglutaminase – coagulation factor XIII (FXIII) – has PDI activity as well. PDI activity was measured by determining the ability to renature reduced-denatured RNase (rdRNase). We found that FXIII can re-nature rdRNase, with efficiency comparable to commercial PDI. This PDI activity was inhibited by bacitracin. Like tissue transglu-taminase, FXIII-mediated PDI activity is independent of its transglutaminase activity and is located on the A subunit. Surface-associated PDI has been previously shown to catalyse two distinct functions: transnitrosation with subsequent release of intracellular nitric oxide and disulfide bond rearrangement during platelet integrin ligation. Our results imply that FXIII-PDI activity may have a role in platelet function.

Keywords

Factor XIII - PDI - transglutaminase

Full Text

References

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