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Thromb Haemost 2010; 104(06): 1184-1192
DOI: 10.1160/TH10-05-0308
Platelets and Blood Cells
Schattauer GmbH

Platelets enhance lymphocyte adhesion and infiltration into arterial thrombus

Hu Hu
1   Department of Pathology and Pathophysiology, Zhejiang University School of Medicine, Hangzhou, China
,
Linjing Zhu
1   Department of Pathology and Pathophysiology, Zhejiang University School of Medicine, Hangzhou, China
2   Karolinska Institute, Department of Medicine, Clinical Pharmacology Unit, Karolinska University Hospital (Solna), Stockholm, Sweden
,
Zhangsen Huang
1   Department of Pathology and Pathophysiology, Zhejiang University School of Medicine, Hangzhou, China
,
Qiushang Ji
3   Department of Cardiology, Qilu Hospital of Shandong University, Jinan, China
,
Madhumita Chatterjee
2   Karolinska Institute, Department of Medicine, Clinical Pharmacology Unit, Karolinska University Hospital (Solna), Stockholm, Sweden
,
Wei Zhang
2   Karolinska Institute, Department of Medicine, Clinical Pharmacology Unit, Karolinska University Hospital (Solna), Stockholm, Sweden
,
Nailin Li
1   Department of Pathology and Pathophysiology, Zhejiang University School of Medicine, Hangzhou, China
2   Karolinska Institute, Department of Medicine, Clinical Pharmacology Unit, Karolinska University Hospital (Solna), Stockholm, Sweden
› Author Affiliations Financial support:This work was supported by grants from the Swedish Research Council, the Swedish Heart-Lung Foundation, the National Natural Science Foundation of China (30700267), the Natural Science Foundation of Zhejiang Province (Y206095), the Karolinska Institute, the Swedish Society of Medicine, and the Stockholm County Council.
Further Information

Publication History

Received: 20 May 2010

Accepted after major revision: 02 August 2010

Publication Date:
24 November 2017 (online)

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Summary

Lymphocytes are present in atherosclerotic lesion. We hypothesise that platelets may facilitate lymphocyte infiltration into the arterial wall. Reconstituted human blood or whole blood was perfused through a collagen-coated parallel-plate flow chamber at different shear rates. Adhered platelets markedly enhanced lymphocyte adhesion that increased lymphocyte deposition from 10 ± 3 cells/mm2 of platelet-depleted blood to 38 ± 11 cells/mm2 of platelet-containing blood at the arterial shear rate of 500 s-1. Platelet-dependent lymphocyte adhesion was inhibited by P-selectin, CD40L, and GPIIb/IIIa-blocking agents, suggesting the involvement of multiple adhesion molecules in this heterotypic interaction. Lymphocyte deposition was more marked among T cells, and seen in both small and large cells. B and natural killer cell adhesion was, however, mainly seen in small cells. Platelet-conjugation facilitated lymphocyte adhesion, as suggested by the selective deposition of platelet-conjugated lymphocytes. In a mouse model of arterial thrombosis, FeCl3-induced thrombus formation markedly enhanced lymphocyte adhesion and infiltration into platelet thrombi, which was abolished by GPIIb/IIIa inhibition. In conclusion, platelets support lymphocyte adhesion under arterial flow conditions, which is selective among T cells and involves multiple adhesion molecules. Our data imply that platelets may facilitate the recruitment of circulating lymphocytes at the arterial injured sites.

Keywords

Platelets - lymphocytes - platelet-lymphocyte conjugates - arterial flow - thrombosis
 

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