Expression and localisation of vascular ribonucleases in endothelial cells

Silvia Fischer; Miwako Nishio; Sara Dadkhahi; Julia Gansler; Mona Saffarzadeh; Aya Shibamiyama; Nicolé Kral; Nelli Baal; Takatoshi Koyama; Elisabeth Deindl; Klaus T. Preissner

doi:10.1160/TH10-06-0345

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2011; 105(02): 345-355
DOI: 10.1160/TH10-06-0345

Endothelium and Vascular Development

Schattauer GmbH

Expression and localisation of vascular ribonucleases in endothelial cells

Authors

Silvia Fischer^*

¹Department of Biochemistry, Justus-Liebig-University, Giessen, Germany
Miwako Nishio^*

²Department of Health Care Sciences, Tokyo Medical and Dental University, Tokyo, Japan
Sara Dadkhahi

¹Department of Biochemistry, Justus-Liebig-University, Giessen, Germany
Julia Gansler

¹Department of Biochemistry, Justus-Liebig-University, Giessen, Germany
Mona Saffarzadeh

¹Department of Biochemistry, Justus-Liebig-University, Giessen, Germany
Aya Shibamiyama

¹Department of Biochemistry, Justus-Liebig-University, Giessen, Germany
Nicolé Kral

¹Department of Biochemistry, Justus-Liebig-University, Giessen, Germany
Nelli Baal

³Department of Immunology and Transfusion Medicine, Justus-Liebig-University, Giessen, Germany
Takatoshi Koyama

²Department of Health Care Sciences, Tokyo Medical and Dental University, Tokyo, Japan
Elisabeth Deindl

⁴Walter-Brendel-Centre of Experimental Medicine, Ludwig-Maximilians-University, Munich, Germany
Klaus T. Preissner

¹Department of Biochemistry, Justus-Liebig-University, Giessen, Germany

Abstract

Summary

The functions of extracellular RNA in the vascular system as new procoagulatory and permeability-increasing factor in vivoand in vitrowere shown to be counteracted by pancreatic type RNase1. Based on the identification of RNase1 in plasma and serum, it is proposed that the enzyme is expressed by vascular cells to contribute in the regulation of extracellular RNA. It is demonstrated that RNase1 and RNase5 (also termed angiogenin) were differentially expressed in various types of endothelial cells, whereby human umbilical vein endothelial cells (HUVEC) expressed and released the highest concentration of active RNase1. Expression and release of RNase5 were similar in all types of endothelial cells tested. Both RNases were constitutively produced and secreted, whereby a portion of RNase1, but not RNase5, was stored in Weibel-Palade bodies, co-localising with von Willlebrand factor and P-selectin. Accordingly, immediate release of RNase1 from these granules was demonstrated in vitroand in vivousing Weibel-Palade body exocytosis-inducing agents. Additionally, extracellular RNA or poly:IC (but not DNA) induced this short-term release of RNase1. Our results indicate that vascular RNase1 and RNase5 are mainly produced by vascular endothelial cells and can serve, depending on the vascular bed, different functions in vascular homeostasis and endothelial cell responses.

Keywords

Human pancreatic RNase1 - angiogenin - endothelial cells - WeibelPalade bodies

Full Text

References

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