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Thromb Haemost 2011; 105(02): 345-355
DOI: 10.1160/TH10-06-0345
Endothelium and Vascular Development
Schattauer GmbH

Expression and localisation of vascular ribonucleases in endothelial cells

Silvia Fischer   *
1   Department of Biochemistry, Justus-Liebig-University, Giessen, Germany
,
Miwako Nishio   *
2   Department of Health Care Sciences, Tokyo Medical and Dental University, Tokyo, Japan
,
Sara Dadkhahi
1   Department of Biochemistry, Justus-Liebig-University, Giessen, Germany
,
Julia Gansler
1   Department of Biochemistry, Justus-Liebig-University, Giessen, Germany
,
Mona Saffarzadeh
1   Department of Biochemistry, Justus-Liebig-University, Giessen, Germany
,
Aya Shibamiyama
1   Department of Biochemistry, Justus-Liebig-University, Giessen, Germany
,
Nicolé Kral
1   Department of Biochemistry, Justus-Liebig-University, Giessen, Germany
,
Nelli Baal
3   Department of Immunology and Transfusion Medicine, Justus-Liebig-University, Giessen, Germany
,
Takatoshi Koyama
2   Department of Health Care Sciences, Tokyo Medical and Dental University, Tokyo, Japan
,
Elisabeth Deindl
4   Walter-Brendel-Centre of Experimental Medicine, Ludwig-Maximilians-University, Munich, Germany
,
Klaus T. Preissner
1   Department of Biochemistry, Justus-Liebig-University, Giessen, Germany
› Author Affiliations Financial support: Studies were generously supported by grants (SFB 547, SPP 1190, FI 543/2–1) and the Excellence cluster “Cardio-pulmonary System” (ECCPS) and the IRTG-1566 (PROMISE) from the Deutsche Forschungsgemeinschaft (DFG, Bonn, Germany) received by Klaus T. Preissner and Silvia Fischer.
Further Information

Publication History

Received: 08 June 2010

Accepted after major revision: 05 November 2010

Publication Date:
25 November 2017 (online)

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Summary

The functions of extracellular RNA in the vascular system as new procoagulatory and permeability-increasing factor in vivoand in vitrowere shown to be counteracted by pancreatic type RNase1. Based on the identification of RNase1 in plasma and serum, it is proposed that the enzyme is expressed by vascular cells to contribute in the regulation of extracellular RNA. It is demonstrated that RNase1 and RNase5 (also termed angiogenin) were differentially expressed in various types of endothelial cells, whereby human umbilical vein endothelial cells (HUVEC) expressed and released the highest concentration of active RNase1. Expression and release of RNase5 were similar in all types of endothelial cells tested. Both RNases were constitutively produced and secreted, whereby a portion of RNase1, but not RNase5, was stored in Weibel-Palade bodies, co-localising with von Willlebrand factor and P-selectin. Accordingly, immediate release of RNase1 from these granules was demonstrated in vitroand in vivousing Weibel-Palade body exocytosis-inducing agents. Additionally, extracellular RNA or poly:IC (but not DNA) induced this short-term release of RNase1. Our results indicate that vascular RNase1 and RNase5 are mainly produced by vascular endothelial cells and can serve, depending on the vascular bed, different functions in vascular homeostasis and endothelial cell responses.

Keywords

Human pancreatic RNase1 - angiogenin - endothelial cells - WeibelPalade bodies

* These authors contributed equally to this study.


 

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