Mass spectrometry for the evaluation of cardiovascular diseases based on proteomics and lipidomics

Aurélien Thomas; Sébastien Lenglet; Pierre Chaurand; Julien Déglon; Patrice Mangin; François Mach; Sabine Steffens; Jean-Luc Wolfender; Christian Staub

doi:10.1160/TH10-12-0812

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2011; 106(01): 20-33
DOI: 10.1160/TH10-12-0812

Review Article

Schattauer GmbH

Mass spectrometry for the evaluation of cardiovascular diseases based on proteomics and lipidomics

Aurélien Thomas

¹Unit of Toxicology, CURML, Geneva University Hospitals, Geneva, Switzerland

⁵Swiss Center of Applied Human Toxicology, University of Geneva, Geneva, Switzerland

,

Sébastien Lenglet

²Division of Cardiology, Department of Internal Medicine, University Hospital, Foundation for Medical Researches, Geneva, Switzerland

,

Pierre Chaurand

³Department of Chemistry, Université de Montréal, Montreal, Quebec, Canada

,

Julien Déglon

¹Unit of Toxicology, CURML, Geneva University Hospitals, Geneva, Switzerland

⁵Swiss Center of Applied Human Toxicology, University of Geneva, Geneva, Switzerland

,

Patrice Mangin

¹Unit of Toxicology, CURML, Geneva University Hospitals, Geneva, Switzerland

,

François Mach

²Division of Cardiology, Department of Internal Medicine, University Hospital, Foundation for Medical Researches, Geneva, Switzerland

,

Sabine Steffens

²Division of Cardiology, Department of Internal Medicine, University Hospital, Foundation for Medical Researches, Geneva, Switzerland

,

Jean-Luc Wolfender

⁴School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Geneva, Switzerland

,

Christian Staub

¹Unit of Toxicology, CURML, Geneva University Hospitals, Geneva, Switzerland

⁵Swiss Center of Applied Human Toxicology, University of Geneva, Geneva, Switzerland

› Author Affiliations

Abstract

Summary

The identification and quantification of proteins and lipids is of major importance for the diagnosis, prognosis and understanding of the molecular mechanisms involved in disease development. Owing to its selectivity and sensitivity, mass spectrometry has become a key technique in analytical platforms for proteomic and lipidomic investigations. Using this technique, many strategies have been developed based on unbiased or targeted approaches to highlight or monitor molecules of interest from biomatrices. Although these approaches have largely been employed in cancer research, this type of investigation has been met by a growing interest in the field of cardiovascular disorders, potentially leading to the discovery of novel biomarkers and the development of new therapies. In this paper, we will review the different mass spectrometry- based proteomic and lipidomic strategies applied in cardiovascular diseases, especially atherosclerosis. Particular attention will be given to recent developments and the role of bioinformatics in data treatment. This review will be of broad interest to the medical community by providing a tutorial of how mass spectrometric strategies can support clinical trials.

Keywords

Mass spectrometry - proteomics - lipidomics - cardiovascular diseases - imaging mass spectrometry

Full Text

References

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