Variability of fibrinogen measurements in post-myocardial infarction patients

Jens Baumert; Mahir Karakas; Sonja Greven; Regina Rückerl; Annette Peters; Wolfgang Koenig

doi:10.1160/TH11-10-0703

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2012; 107(05): 895-902
DOI: 10.1160/TH11-10-0703

Blood Coagulation, Fibrinolysis and Cellular Haemostasis

Schattauer GmbH

Variability of fibrinogen measurements in post-myocardial infarction patients

Results from the AIRGENE study center Augsburg

Authors

Jens Baumert

¹Institute of Epidemiology II, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany
Mahir Karakas

²Department of Internal Medicine II -Cardiology, University of Ulm Medical Center, Ulm, Germany
Sonja Greven

³Department of Statistics, Ludwig-Maximilians-Universität München, Munich, Germany
Regina Rückerl

¹Institute of Epidemiology II, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany
Annette Peters

¹Institute of Epidemiology II, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany
Wolfgang Koenig

²Department of Internal Medicine II -Cardiology, University of Ulm Medical Center, Ulm, Germany

Abstract

Summary

Elevated fibrinogen levels are strongly and consistently associated with incident coronary heart disease (CHD). A possible causal contribution of fibrinogen in the pathway leading to atherothrombotic cardiovascular disease complications has been suggested. However, for implementation in clinical practice, data on validity and reliability, which are still scarce, are needed that are still scarce, especially in subjects with a history of CHD. For the present study, levels of plasma fibrinogen were measured in 200 post-myocardial infarction (post-MI) patients aged 39–76 years, with approximately six blood samples collected at monthly intervals between May 2003 and March 2004, giving a total of 1,144 samples. Inter-individual variability (between-subject variance component, VC_b and coefficient of variation, CV_b), intra-individual and analytical variability (VC_w+a and CV_w+a), intraclass correlation coefficient (ICC) and the number of measurements required for an ICC of 0.75 were estimated to assess the reliability of serial fibrinogen measurements. Mean fibrinogen concentration of all subjects over all samples was 3.34 g/l (standard deviation 0.67). Between-subject variation for fibrinogen was VC_b = 0.34 (CV_b,=17.5%) whereas within-subject and analytical variation was estimated as VC_w+a = 0.14 (CV_w+a=11.0%). The variation was mainly explained by between-subject variability, shown by the proportion of total variance of 71.3%. Two different measurements were required to reach sufficient reliability, if subjects with extreme values were not excluded. The present study indicates a fairly good reproducibility of serial individual fibrinogen measurements in post-MI subjects.

Keywords

Myocardial infarction - fibrinogen - cardiovascular risk factors - biological variation - number of measurements

Full Text

References

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