Circulating microparticles in carriers of factor V Leiden with and without a history of venous thrombosis

Elena Campello; Luca Spiezia; Claudia M. Radu; Maria Bon; Sabrina Gavasso; Patrizia Zerbinati; Barry Woodhams; Daniela Tormene; Paolo Prandoni; Paolo Simioni

doi:10.1160/TH12-05-0280

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 2012; 108(04): 633-639
DOI: 10.1160/TH12-05-0280

Blood Coagulation, Fibrinolysis and Cellular Haemostasis

Schattauer GmbH

Circulating microparticles in carriers of factor V Leiden with and without a history of venous thrombosis

Autor*innen

Elena Campello

¹Department of Cardiologic, Thoracic, and Vascular Sciences, 2nd Chair of Internal Medicine, University of Padua Medical School, Padua, Italy
Luca Spiezia

¹Department of Cardiologic, Thoracic, and Vascular Sciences, 2nd Chair of Internal Medicine, University of Padua Medical School, Padua, Italy
Claudia M. Radu

¹Department of Cardiologic, Thoracic, and Vascular Sciences, 2nd Chair of Internal Medicine, University of Padua Medical School, Padua, Italy
Maria Bon

¹Department of Cardiologic, Thoracic, and Vascular Sciences, 2nd Chair of Internal Medicine, University of Padua Medical School, Padua, Italy
Sabrina Gavasso

¹Department of Cardiologic, Thoracic, and Vascular Sciences, 2nd Chair of Internal Medicine, University of Padua Medical School, Padua, Italy
Patrizia Zerbinati

¹Department of Cardiologic, Thoracic, and Vascular Sciences, 2nd Chair of Internal Medicine, University of Padua Medical School, Padua, Italy
Barry Woodhams

²Diagnostica Stago, Gennevilliers, France
Daniela Tormene

¹Department of Cardiologic, Thoracic, and Vascular Sciences, 2nd Chair of Internal Medicine, University of Padua Medical School, Padua, Italy
Paolo Prandoni

¹Department of Cardiologic, Thoracic, and Vascular Sciences, 2nd Chair of Internal Medicine, University of Padua Medical School, Padua, Italy
Paolo Simioni

¹Department of Cardiologic, Thoracic, and Vascular Sciences, 2nd Chair of Internal Medicine, University of Padua Medical School, Padua, Italy

Abstract

Summary

Although factor V Leiden (FVL) is a major determinant of thrombotic risk, the reason why less than 10% of carriers eventually develop venous thromboembolic (VTE) events is unknown. Recent observations suggest that circulating levels of microparticles (MP) may contribute to the thrombogenic profile of FVL carriers. We measured the plasma level of annexin V-MP (AMP) platelet-MP (PMP), endothelial-MP (EMP), leukocyte-MP (LMP) and tissue factor-bearing MP (TF⁺MP), and the MP procoagulant activity (PPL) in 142 carriers of FVL (of these 30 homozygous and 49 with prior VTE), and in 142 age and gender-matched healthy individuals. The mean (± SD) level of AMP was 2,802 ± 853 MP/ μl in carriers and 1,682 ± 897 in controls (p<0.0001). A statistically significant difference between homozygous and heterozygous carriers of FVL was seen in the level of PMP, EMP and LMP, but not in that of the remaining parameters. When the analysis was confined to carriers with and without a VTE history, the mean level of AMP was 3,110 ± 791 MP/ μl in the former, and 2,615 ± 839 MP/μl in the latter (p<0.005). The mean level of all subtypes of circulating MP showed a similar pattern. The PPL clotting time was 39 ± 9 seconds (sec) in carriers, and 52 ± 15 sec in controls (p=0.003); and was 35 ± 8 sec in carriers with prior thrombosis, and 41 ± 10 sec in thrombosis-free carriers (p<0.005). Our study results suggest that circulating MP may contribute to the development of thrombosis in carriers of FVL mutation.

Keywords

Microparticles - factor V Leiden - tissue factor-bearing microparticles - venous thromboembolism

Volltext

Referenzen

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