Circulating microparticles: challenges and perspectives of flow cytometric assessment

Eduard Shantsila; Silvia Montoro-García; Pilar Gallego; Gregory Y. H. Lip

doi:10.1160/TH13-11-0937

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2014; 111(06): 1009-1014
DOI: 10.1160/TH13-11-0937

Clinical Focus

Schattauer GmbH

Circulating microparticles: challenges and perspectives of flow cytometric assessment

Eduard Shantsila

¹University of Birmingham Centre for Cardiovascular Sciences, City Hospital, Birmingham, UK

,

Silvia Montoro-García

¹University of Birmingham Centre for Cardiovascular Sciences, City Hospital, Birmingham, UK

²Department of Cardiology, Hospital Universitario Virgen de la Arrixaca, University of Murcia, Spain

,

Pilar Gallego

¹University of Birmingham Centre for Cardiovascular Sciences, City Hospital, Birmingham, UK

,

Gregory Y. H. Lip

¹University of Birmingham Centre for Cardiovascular Sciences, City Hospital, Birmingham, UK

› Author Affiliations

Abstract

Summary

Circulating blood microparticles are likely to play a significant role as messengers of biological information. Their accurate quantification and characterisation is challenging and needs to be carefully designed with preferable usage of fresh minimally-processed blood samples. Utilisation of flow cytometers specifically designed for analysis of small-size particles is likely to provide considerable methodological advantages and should be the preferable option. This viewpoint manuscript provides a critical summary of the key methodological aspects of microparticle analysis.

Note: The review process for this viewpoint article was fully handled by Christian Weber, Editor in Chief.

Keywords

Microparticles - flow cytometry - microvesicles

Full Text

References

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