DNA, histones and neutrophil extracellular traps exert anti-fibrinolytic effects in a plasma environment

Imre Varjú; Colin Longstaff; László Szabó; Ádám Zoltán Farkas; Veronika Judit Varga-Szabó; Anna Tanka-Salamon; Raymund Machovich; Krasimir Kolev

doi:10.1160/TH14-08-0669

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2015; 113(06): 1289-1298
DOI: 10.1160/TH14-08-0669

Coagulation and Fibrinolysis

Schattauer GmbH

DNA, histones and neutrophil extracellular traps exert anti-fibrinolytic effects in a plasma environment

Imre Varjú

¹Department of Medical Biochemistry, Semmelweis University, Budapest, Hungary

,

Colin Longstaff

²Biotherapeutics Division, Haemostasis Section, National Institute for Biological Standards and Control, South Mimms, Herts, UK

,

László Szabó

³Department of Functional and Structural Materials, Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary

,

Ádám Zoltán Farkas

¹Department of Medical Biochemistry, Semmelweis University, Budapest, Hungary

,

Veronika Judit Varga-Szabó

¹Department of Medical Biochemistry, Semmelweis University, Budapest, Hungary

,

Anna Tanka-Salamon

¹Department of Medical Biochemistry, Semmelweis University, Budapest, Hungary

,

Raymund Machovich

¹Department of Medical Biochemistry, Semmelweis University, Budapest, Hungary

,

Krasimir Kolev

¹Department of Medical Biochemistry, Semmelweis University, Budapest, Hungary

› Author Affiliations

Abstract

Summary

In response to various inflammatory stimuli, neutrophils secrete neutrophil extracellular traps (NETs), web-like meshworks of DNA, histones and granular components forming supplementary scaffolds in venous and arterial thrombi. Isolated DNA and histones are known to promote thrombus formation and render fibrin clots more resistant to mechanical forces and tissue-type plasminogen activator (tPA)-induced enzymatic digestion. The present study extends our earlier observations to a physiologically more relevant environment including plasma clots and NET-forming neutrophils. A range of techniques was employed including imaging (scanning electron microscopy (SEM), confocal laser microscopy, and photoscanning of macroscopic lysis fronts), clot permeability measurements, turbidimetric lysis and enzyme inactivation assays. Addition of DNA and histones increased the median fibre diameter of plasma clots formed with 16 nM thrombin from 108 to 121 and 119 nm, respectively, and decreased their permeability constant from 6.4 to 3.1 and 3.7×10⁻⁹ cm². Histones effectively protected thrombin from antithrombin-induced inactivation, while DNA inhibited plasminogen activation on the surface of plasma clots and their plasmin-induced resolution by 20 and 40 %, respectively. DNA and histones, as well as NETs secreted by phorbol-myristate-acetate-activated neutrophils, slowed down the tPA-driven lysis of plasma clots and the latter effect could be reversed by the addition of DNase (streptodornase). SEM images taken after complete digestion of fibrin in NET-containing plasma clots evidenced retained NET scaffold that was absent in DNase-treated clots. Our results show that DNA and histones alter the fibrin architecture in plasma clots, while NETs contribute to a decreased lytic susceptibility that can be overcome by DNase.

Keywords

Neutrophil extracellular traps - DNA - histones - fibrinolysis - plasmin

Full Text

References

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