Rapid immunochromatographic test for detection of anti-factor XIII A subunit antibodies can diagnose 90 % of cases with autoimmune haemorrhaphilia XIII/13

Tsukasa Osaki; Daisuke Sugiyama; Yasuo Magari; Masayoshi Souri; Akitada Ichinose

doi:10.1160/TH14-09-0745

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2015; 113(06): 1347-1356
DOI: 10.1160/TH14-09-0745

New Technologies, Diagnostic Tools and Drugs

Schattauer GmbH

Rapid immunochromatographic test for detection of anti-factor XIII A subunit antibodies can diagnose 90 % of cases with autoimmune haemorrhaphilia XIII/13

Tsukasa Osaki

¹Department of Molecular Patho-Biochemistry and Patho-Biology, Yamagata University Faculty of Medicine, Yamagata, Japan

,

Daisuke Sugiyama

²Q-may Laboratory Corporation, Oita, Japan

,

Yasuo Magari

²Q-may Laboratory Corporation, Oita, Japan

,

Masayoshi Souri

¹Department of Molecular Patho-Biochemistry and Patho-Biology, Yamagata University Faculty of Medicine, Yamagata, Japan

,

Akitada Ichinose

¹Department of Molecular Patho-Biochemistry and Patho-Biology, Yamagata University Faculty of Medicine, Yamagata, Japan

› Author Affiliations

Abstract

Summary

Autoimmune haemorrhaphilia XIII/13 (AH13) is an acquired lifethreatening bleeding disorder due to anti-factor XIII (FXIII) autoantibodies (auto-Abs). AH13 patients may die of haemorrhage without correct diagnosis and proper treatment because of lack of awareness and the absence of rapid easy-to-use tests specific for this disease. Currently, the definitive diagnosis is established by cumbersome and time-consuming laboratory tests such as dot-blot assays and enzymelinked immunosorbent assays (ELISA), and therefore these tests are generally not carried out. To save AH13 patients’ lives, there is an urgent necessity for developing a rapid test for FXIII auto-Abs. We first generated and characterised mouse monoclonal antibodies (mAb) against human FXIII A subunit (FXIII-A), and then developed a rapid immunochromatographic test (ICT) for detection of anti-FXIII-A auto- Abs using one mAb with a dissociation constant of 9.3 × 10^-11 M. The auto-Ab-FXIII-A complex was captured by the mAb on a nitrocellulose membrane and visualised by Au-conjugated anti-human IgG Ab. Mixing with healthy control plasma improved the detection of auto-Abs in patients having extremely low levels of FXIII-A. The specificity and sensitivity of the ICT were 87% and 94%, respectively. We also detected auto-Abs against activated FXIII (FXIIIa) in three patients by pre-converting FXIII to FXIIIa by thrombin treatment. ICT values were significantly inversely correlated with FXIII activity levels, indicating an association between the quantity of anti-FXIII autoantibodies and AH13. This reliable rapid ICT assay can be applied to a point-of-care test to detect anti-FXIII-A auto-Abs, and will contribute to early diagnosis and treatment of AH13.

Keywords

Acquired coagulation disorders - autoantibodies - autoimmune diseases - factor XIII / transglutaminases - diagnosis management

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References

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