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Thromb Haemost 2015; 114(06): 1322-1323
DOI: 10.1160/TH15-02-0175
Letter to the Editor
Schattauer GmbH

A modified PF4-dependent, CD62p expression assay selectively detects serotonin-releasing antibodies in patients suspected of HIT

Anand Padmanabhan
1   Medical Sciences Institute, BloodCenter of Wisconsin, Milwaukee, Wisconsin, USA
2   Blood Research Institute, Blood-Center of Wisconsin, Milwaukee, Wisconsin, USA
3   Department of Pathology, Medical College of Wisconsin, Milwaukee, Wisconsin, USA
,
Curtis G. Jones
1   Medical Sciences Institute, BloodCenter of Wisconsin, Milwaukee, Wisconsin, USA
,
Daniel W. Bougie
2   Blood Research Institute, Blood-Center of Wisconsin, Milwaukee, Wisconsin, USA
,
Brian R. Curtis
2   Blood Research Institute, Blood-Center of Wisconsin, Milwaukee, Wisconsin, USA
4   Platelet and Neutrophil Immunology Laboratory, BloodCenter of Wisconsin, Milwaukee, Wisconsin, USA
,
Janice G. McFarland
4   Platelet and Neutrophil Immunology Laboratory, BloodCenter of Wisconsin, Milwaukee, Wisconsin, USA
5   Department of Medicine, Medical College of Wisconsin, Milwaukee, Wisconsin, USA
,
Demin Wang
2   Blood Research Institute, Blood-Center of Wisconsin, Milwaukee, Wisconsin, USA
6   Department of Microbiology & Molecular Genetics, Medical College of Wisconsin, Milwaukee, Wisconsin, USA
,
Richard H. Aster
2   Blood Research Institute, Blood-Center of Wisconsin, Milwaukee, Wisconsin, USA
5   Department of Medicine, Medical College of Wisconsin, Milwaukee, Wisconsin, USA
› Author Affiliations
Further Information

Publication History

Received: 24 February 2015

Accepted after major revision: 05 June 2015

Publication Date:
18 November 2017 (online)

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  • References

  • 1 Shantsila E, Lip GY, Chong BH. Heparin-induced thrombocytopenia. A contemporary clinical approach to diagnosis and management. Chest 2009; 135: 1651-1664.
    PubMedSearch in Google Scholar
  • 2 Lo GK, Sigouin CS, Warkentin TE. What is the potential for overdiagnosis of heparin-induced thrombocytopenia?. Am J Hematol 2007; 82: 1037-1043.
    CrossrefPubMedSearch in Google Scholar
  • 3 Warkentin TE, Levine MN, Hirsh J. et al. Heparin-induced thrombocytopenia in patients treated with low-molecular-weight heparin or unfractionated heparin. N Engl J Med 1995; 332: 1330-1335.
    CrossrefPubMedSearch in Google Scholar
  • 4 Warkentin TE. How I diagnose and manage HIT. Hematology Am Soc Hematol Educ Program 2011; 2011: 143-149.
    CrossrefPubMedSearch in Google Scholar
  • 5 Morel-Kopp MC, Tan CW, Brighton TA. et al. Validation of whole blood impedance aggregometry as a new diagnostic tool for HIT: results of a large Australian study. Thromb Haemost 2012; 107: 575-583.
    Thieme ConnectPubMedSearch in Google Scholar
  • 6 Padmanabhan A, Jones CG, Bougie DW. et al. Heparin-independent, PF4-dependent binding of HIT antibodies to platelets: implications for HIT pathogenesis. Blood 2015; 125: 155-161.
    CrossrefPubMedSearch in Google Scholar
  • 7 Hicks LK, Bering H, Carson KR. et al. Five hematologic tests and treatments to question. Blood 2014; 124: 3524-3528.
    CrossrefPubMedSearch in Google Scholar
  • 8 Jy W, Mao WW, Horstman LL. et al. A flow cyto-metric assay of platelet activation marker P-selectin (CD62P) distinguishes heparin-induced thrombocytopenia (HIT) from HIT with thrombosis (HITT). Thromb Haemost 1999; 82: 1255-1259.
    Thieme ConnectPubMedSearch in Google Scholar
  • 9 Vitale M, Tazzari P, Ricci F. et al. Comparison between different laboratory tests for the detection and prevention of heparin-induced thrombocytopenia. Cytometry 2001; 46: 290-295.
    CrossrefPubMedSearch in Google Scholar
  • 10 Cuker A, Arepally G, Crowther MA. et al. The HIT Expert Probability (HEP) Score: a novel pre-test probability model for heparin-induced thrombocytopenia based on broad expert opinion. J Thromb Haemost 2010; 08: 2642-2650.
    CrossrefPubMedSearch in Google Scholar