Oxidative stress and thromboxane-dependent platelet activation in inflammatory bowel disease: effects of anti-TNF-α treatment

Antonio Di Sabatino; Francesca Santilli; Marco Guerci; Paola Simeone; Sandro Ardizzone; Alessandro Massari; Paolo Giuffrida; Romina Tripaldi; Alessandro Malara; Rossella Liani; Evelyn Gurini; Nicola Aronico; Alessandra Balduini; Gino Roberto Corazza; Giovanni Davì

doi:10.1160/TH16-02-0167

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 2016; 116(03): 486-495
DOI: 10.1160/TH16-02-0167

Cellular Haemostasis and Platelets

Schattauer GmbH

Oxidative stress and thromboxane-dependent platelet activation in inflammatory bowel disease: effects of anti-TNF-α treatment

Autor*innen

Antonio Di Sabatino

²First Department of Internal Medicine and Biotechnology Laboratories
Francesca Santilli

¹Internal Medicine and Center of Excellence on Aging, “G. d’Annunzio” University of Chieti-Pescara, Chieti, Italy
Marco Guerci

²First Department of Internal Medicine and Biotechnology Laboratories
Paola Simeone

¹Internal Medicine and Center of Excellence on Aging, “G. d’Annunzio” University of Chieti-Pescara, Chieti, Italy
Sandro Ardizzone

⁴Division of Gastroenterology, “L. Sacco” University Hospital, Milan, Italy
Alessandro Massari

⁴Division of Gastroenterology, “L. Sacco” University Hospital, Milan, Italy
Paolo Giuffrida

²First Department of Internal Medicine and Biotechnology Laboratories
Romina Tripaldi

¹Internal Medicine and Center of Excellence on Aging, “G. d’Annunzio” University of Chieti-Pescara, Chieti, Italy
Alessandro Malara

³Department of Molecular Medicine, University of Pavia, IRCCS San Matteo Foundation, Pavia, Italy
Rossella Liani

¹Internal Medicine and Center of Excellence on Aging, “G. d’Annunzio” University of Chieti-Pescara, Chieti, Italy
Evelyn Gurini

³Department of Molecular Medicine, University of Pavia, IRCCS San Matteo Foundation, Pavia, Italy
Nicola Aronico

²First Department of Internal Medicine and Biotechnology Laboratories
Alessandra Balduini

³Department of Molecular Medicine, University of Pavia, IRCCS San Matteo Foundation, Pavia, Italy
Gino Roberto Corazza

²First Department of Internal Medicine and Biotechnology Laboratories
Giovanni Davì

¹Internal Medicine and Center of Excellence on Aging, “G. d’Annunzio” University of Chieti-Pescara, Chieti, Italy

Abstract

Summary

Patients with inflammatory bowel disease (IBD) are at higher risk of venous thromboembolism and coronary artery disease despite having a lower burden of traditional risk factors. Platelets from IBD patients release more soluble CD40 ligand (CD40L), and this has been implicated in IBD platelet hyper-activation. We here measured the urinary F₂-isoprostane 8-iso-prostaglandin (PG)_2α (8-iso-PGF_2α), urinary 11–dehydro–thromboxane (TX) B₂ (11-dehydro–TXB₂) and plasma CD40L in IBD patients, and explored the in vitro action of anti-tumour necrosis factor (TNF)–α antibody infliximab on IBD differentiating megakaryocytes. Urinary and blood samples were collected from 124 IBD patients and 37 healthy subjects. Thirteen IBD patients were also evaluated before and after 6–week infliximab treatment. The in vitro effect of infliximab on patient-derived megakaryocytes was evaluated by immunoflorescence microscopy and by flow cytometry. IBD patients had significantly (p<0.0001) higher urinary 8–iso–PGF_2α and 11–dehydro–TXB₂ as well as plasma CD40L levels than controls, with active IBD patients displaying higher urinary and plasma values when compared to inactive patients in remission. A 6-week treatment with infliximab was associated with a significant reduction of the urinary excretion of 8–iso–PGF_2α and 11–dehydro–TXB₂ (p=0.008) and plasma CD40L (p=0.001). Infliximab induced significantly rescued pro-platelet formation by megakaryocytes derived from IBD patients but not from healthy controls. Our findings provide evidence for enhanced in vivo TX–dependent platelet activation and lipid peroxidation in IBD patients. Anti-TNF–α therapy with infliximab down-regulates in vivo isoprostane generation and TX biosynthesis in responder IBD patients. Further studies are needed to clarify the implication of infliximab induced-proplatelet formation from IBD megakaryocytes.

Supplementary Material to this article is available online at www.thrombosis-online.com.

Keywords

CD40 ligand - Crohn’s disease - infliximab - lipid peroxidation - platelet activation - ulcerative colitis

Volltext

Referenzen

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