CCR5+ CD8 T-cell levels and monocyte activation precede the onset of acute coronary syndrome in HIV-infected patients on antiretroviral therapy

Laura Tarancon-Diez; Rebeca S. De Pablo-Bernal; Ana I. Alvarez-Ríos; Isaac Rosado-Sánchez; Beatriz Dominguez-Molina; Miguel Genebat; Yolanda M. Pacheco; José Luis Jiménez; M. Ángeles Muñoz-Fernández; Ezequiel Ruiz-Mateos; Manuel Leal

doi:10.1160/TH16-11-0867

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 2017; 117(06): 1141-1149
DOI: 10.1160/TH16-11-0867

Blood Cells, Inflammation and Infection

Schattauer GmbH

CCR5+ CD8 T-cell levels and monocyte activation precede the onset of acute coronary syndrome in HIV-infected patients on antiretroviral therapy

Laura Tarancon-Diez^*

¹Laboratory of Immunovirology, Biomedicine Institute of Seville, Virgen del Rocío University Hospital, IBIS/CSIC/SAS/University of Seville, Seville, Spain

²Biochemistry Department, Virgen del Rocío University Hospital, Seville, Spain

,

Rebeca S. De Pablo-Bernal^*

¹Laboratory of Immunovirology, Biomedicine Institute of Seville, Virgen del Rocío University Hospital, IBIS/CSIC/SAS/University of Seville, Seville, Spain

²Biochemistry Department, Virgen del Rocío University Hospital, Seville, Spain

,

Ana I. Alvarez-Ríos

¹Laboratory of Immunovirology, Biomedicine Institute of Seville, Virgen del Rocío University Hospital, IBIS/CSIC/SAS/University of Seville, Seville, Spain

²Biochemistry Department, Virgen del Rocío University Hospital, Seville, Spain

,

Isaac Rosado-Sánchez

¹Laboratory of Immunovirology, Biomedicine Institute of Seville, Virgen del Rocío University Hospital, IBIS/CSIC/SAS/University of Seville, Seville, Spain

²Biochemistry Department, Virgen del Rocío University Hospital, Seville, Spain

,

Beatriz Dominguez-Molina

¹Laboratory of Immunovirology, Biomedicine Institute of Seville, Virgen del Rocío University Hospital, IBIS/CSIC/SAS/University of Seville, Seville, Spain

²Biochemistry Department, Virgen del Rocío University Hospital, Seville, Spain

,

Miguel Genebat

¹Laboratory of Immunovirology, Biomedicine Institute of Seville, Virgen del Rocío University Hospital, IBIS/CSIC/SAS/University of Seville, Seville, Spain

²Biochemistry Department, Virgen del Rocío University Hospital, Seville, Spain

,

Yolanda M. Pacheco

¹Laboratory of Immunovirology, Biomedicine Institute of Seville, Virgen del Rocío University Hospital, IBIS/CSIC/SAS/University of Seville, Seville, Spain

²Biochemistry Department, Virgen del Rocío University Hospital, Seville, Spain

,

José Luis Jiménez

³Molecular Immunobiology Laboratory, General Universitary Hospital Gregorio Marañon, Health Research Institute Gregorio Marañon, Spanish HIV HGM BioBank, Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid, Spain

⁴Viral and Immune Infection Unit Center, Institute of Health Carlos III, Majadahonda Campus, Molecular Immunobiology Laboratory, General Universitary Hospital Gregorio Marañon, Madrid, Spain

,

M. Ángeles Muñoz-Fernández

³Molecular Immunobiology Laboratory, General Universitary Hospital Gregorio Marañon, Health Research Institute Gregorio Marañon, Spanish HIV HGM BioBank, Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid, Spain

⁴Viral and Immune Infection Unit Center, Institute of Health Carlos III, Majadahonda Campus, Molecular Immunobiology Laboratory, General Universitary Hospital Gregorio Marañon, Madrid, Spain

,

Ezequiel Ruiz-Mateos⁺

¹Laboratory of Immunovirology, Biomedicine Institute of Seville, Virgen del Rocío University Hospital, IBIS/CSIC/SAS/University of Seville, Seville, Spain

²Biochemistry Department, Virgen del Rocío University Hospital, Seville, Spain

,

Manuel Leal⁺

¹Laboratory of Immunovirology, Biomedicine Institute of Seville, Virgen del Rocío University Hospital, IBIS/CSIC/SAS/University of Seville, Seville, Spain

²Biochemistry Department, Virgen del Rocío University Hospital, Seville, Spain

› Institutsangaben

Abstract

Summary

Acute coronary syndrome (ACS) is nowadays one of the leading causes of morbid-mortality in HIV-infected population, but innate and adaptive immune mechanisms preceding this event are unknown. In this work we comprehensively and longitudinally observed, by multi-parametric flow cytometry and following a case-control design, increased CCR5⁺CD8⁺ T-cells levels and monocytes expressing activation and adhesion markers in HIV-infected patients who are going to suffer ACS. In addition, we found direct associations between activated CD8⁺ T-cells and myeloid cells that were only statistically significant in the group of patients with ACS and in the follow up time point just before the ACS. Our data highlight the important role of CCR5 in the onset of ACS and suggest this receptor as a marker of cardiovascular risk and potential therapeutic target to prevent the development of such non-AIDS-related event in HIV-infected patients.

Note: This work was presented at the CROI 2016 conference (Boston, USA in February 2016).

Keywords

HIV - CCR5 - monocyte - vascular event - immune activation

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Referenzen

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