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Tierarztl Prax Ausg K Kleintiere Heimtiere 2016; 44(02): 118-122
DOI: 10.15654/TPK-150484
Kasuistik
Schattauer GmbH

Bocavirus-Nachweis bei 4 Wochen alten Welpen mit akutem Durchfall

Detection of bocavirus in 4-week-old puppies with acute diarrhea
S. Rudolf
1   Klinikum Veterinärmedizin, Klinik für Kleintiere, Innere Medizin, Justus-Liebig-Universität Gießen
,
R. Neiger
1   Klinikum Veterinärmedizin, Klinik für Kleintiere, Innere Medizin, Justus-Liebig-Universität Gießen
,
M. König
2   Institut für Virologie, Fachbereich Veterinärmedizin der Justus-Liebig-Universität Gießen
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Weitere Informationen

Publikationsverlauf

Received: 21. Juni 2015

Accepted after major revision: 29. März 2015

Publikationsdatum:
20. Dezember 2017 (online)

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Zusammenfassung

Zwei 4 Wochen alte, weibliche Dobermann-Welpen wurden mit akutem Durchfall und Erbrechen vorgestellt. Zusätzlich zeigten sie Niesen und Nasenausfluss. Aufgrund der klinischen Präsentation und einer Neutropenie bestand der hochgradige Verdacht einer Parvovirose. Die Tiere blieben mehrere Tage hospitalisiert und wurden symptomatisch therapiert. Eine parasitologische Kotuntersuchung sowie eine virologische Untersuchung des Kots auf eine Parvovirusinfektion (Polymerasekettenreaktion [PCR] und Immunelektronenmikroskopie) verliefen negativ. Mithilfe einer weiteren, neuen PCR ließ sich das Bocavirus identifizieren, sodass eine Infektion mit diesem Erreger als mögliche Diagnose infrage kommt. Dieser Fallbericht beschreibt eine noch wenig bekannte virale Welpenerkrankung mit erfolgreichem Therapieverlauf.

Summary

Two 4-month-old female Doberman puppies were presented with clinical signs of acute diarrhea and emesis. They also showed sneezing and nasal discharge. The clinical presentation and neutropenia were suggestive of a parvovirus infection. The puppies were hospitalized for several days and treated symptomatically. Fecal samples tested negative for parasites. Virological examination of feces using polymerase chain reaction (PCR) and immune electron microscopy failed to confirm a parvovirus infection. With a recently developed PCR, bocavirus could be identified, thus making an infection with this virus a possible diagnosis. This case report presents a less well-known viral puppy disease and its successful therapy.

Schlüsselwörter

Hund - Neutropenie - Nasenausfluss - Bocavirus - PCR

Keywords

Dog - neutropenia - nasal discharge - bocavirus - PCR
 

  • Literatur

  • 1 Axthelm MK, Krakowka S. Canine distemper virus-induced thrombocytopenia. Am J Vet Res 1987; 48: 1269-1275.
    PubMedGoogle Scholar
  • 2 Bodewes R, Lapp S, Hahn K, Habierski A, Förster C, König M, Wohlsein P, Osterhaus A, Baumgärtner W. Novel canine bocavirus strain associated with severe enteritis in a dog litter. Vet Microbiol 2014; 174: 1-8.
    CrossrefPubMedGoogle Scholar
  • 3 Brown MR, Rogers KS. Neutropenia in dogs and cats: a retrospective study of 261 cases. J Am Anim Hosp Assoc 2001; 37: 131-139.
    CrossrefPubMedGoogle Scholar
  • 4 Buonavoglia C, Martella V, Pratelli A, Tempesta M, Cavalli A, Buonavoglia D, Bozzo G, Elia G, Decaro N, Carmichael LE. Evidence for evolution of canine parvovirus type-2 in Italy. J Gen Virol 2001; 82: 3021-3025.
    CrossrefPubMedGoogle Scholar
  • 5 Carmichael LE, Schlafer DH, Hashimoto A. Minute virus of canine (MCV, canine parvovirus type-1): pathogeneticity for pubs and seroprevalence estimate. J Vet Diagn Invest 1994; 6: 165-174.
    CrossrefPubMedGoogle Scholar
  • 6 Castro TX, Cubel Garcia RC, Costa EM, Leal RM, Xavier Mda P, Leite JP. Molecular characterisation of calicivirus and astrovirus in puppies with enteritis. Vet Rec 2013; 172: 557.
    PubMedGoogle Scholar
  • 7 Decaro N, Amorisco F, Lenoci D, Lovero A, Colaianni ML, Losurdo M, Desario C, Martella V, Buonavoglia C. Molecular chararcterization of Canine minute virus associated with neonatal mortality in a litter of Jack Russel terrier dogs. J Vet Diagn Invest 2012; 24 (04) 755-758.
    CrossrefPubMedGoogle Scholar
  • 8 Decaro N, Buonavoglia C. Canine parvovirus - A review of epidemiological and diagnostic aspects, with emphasis on type 2c. Vet Microbiol 2012; 155: 1-12.
    CrossrefPubMedGoogle Scholar
  • 9 Decaro N, Martella V, Desarion C, Lanave G, Circella E, Cavalli A, Elia G, Camero M, Buonavoglia C. Genomic Characterization of Circovirus Associated with Fatal Hemorrhagic Enteritis in Dog, Italy. Plos one 2014; 9 (08) e105909.
    CrossrefPubMedGoogle Scholar
  • 10 Greene CE, Decaro N. Canine viral enteritis. Infectious Disease of the Dog and Cat. 4th edn. St. Louis, Missouri: Elsevier; 2011: 67-75.
    Google Scholar
  • 11 Harrison LR, Styer EL, Pursell AR. Fatal disease in nursing puppies associated with minute virus of canine. J Vet Diagn Invest 1992; 4: 19-22.
    CrossrefPubMedGoogle Scholar
  • 12 Hengst M, Häusler M, Honnef D, Scheithauer S, Ritter K, Kleines M. Infektion mit dem humanem Bocavirus (hBov): Eine wichtige Ursache schwerer viraler Atemwegsinfektionen im frühen Kindesalter. Klin Pädiatr 2008; 220: 296-301.
    Artikel in Thieme ConnectPubMedGoogle Scholar
  • 13 Kappor A, Mehta N, Dubovi EJ, Simmonds P, Govindasamy L, Medina JL, Street C, Shields S, Lipkin WI. Characterization of novel canine bocaviruses and their association with respiratory disease. J Gen Virol 2012; 93: 341-346.
    CrossrefPubMedGoogle Scholar
  • 14 Kapil S, Cooper E, Lamm C, Murray B, Rezabek G, Johnstron 3rd L, Campbell G, Johnson B. Canine parvovirus types 2c and 2b circulation in North American dogs in 2006 and 2007. J Clin Microbiol 2007; 45 (12) 4044-4047.
    CrossrefPubMedGoogle Scholar
  • 15 Lau SK, Woo PC, Yeung HC, Teng JL, Wu Y, Bai R, Fan RY, Chan KH, Yuen KY. Identification and characterization of bocaviruses in cats and dogs reveals a novel feline bocavirus and a novel genetic group of canine bocavirus. J Gen Virol 2012; 93: 1573-1582.
    CrossrefPubMedGoogle Scholar
  • 16 Manteufel J, Truyen U. Animal bocaviruses: a brief review. Intervirology 2008; 51: 328-334.
    CrossrefPubMedGoogle Scholar
  • 17 Martin V, Najbar W, Guegen S, Grousson D, Eun HM, Lebreux B, Aubert A. Treatment of canine parvoviral enteritis with interferon omega in a placebo-controlled challenge trial. Vet Microbiol 2002; 89: 115-127.
    CrossrefPubMedGoogle Scholar
  • 18 Mohr AJ, Leisewitz AL, Jacobson LS, Steiner JM, Ruaux CG, Williams DA. Effect of early enteral nutrition on intestinal permeability, intestinal protein loss, and outcome in dogs with severe parvoviral enteritis. J Vet Intern Med 2003; 17: 791-798.
    CrossrefPubMedGoogle Scholar
  • 19 Mylonakis ME, Koutinas AF, Breitschwerdt EB, Hegarty BC, Billinis CD, Leontides LS, Kontos VS. Chronic Canine Ehrlichiosis (Ehrlichia canis). J Am Anim Hosp Assoc 2004; 40 (03) 174-184.
    CrossrefPubMedGoogle Scholar
  • 20 Ntafis V, Xylouri E, Radogna A, Buonavoglia C, Martella V. Outbreak of canine norovirus infection in young dogs. J Clin Microbiol 2010; 48 (07) 2605-2608.
    CrossrefPubMedGoogle Scholar
  • 21 Ohshima T, Kawakami K, Abe T, Mochizuki M. A minute virus of canines (MCV: canine bocavirus) isolated from an elderly dog with severe gastroenteritis, and phylogenetic analyses of MCV strains. Elsevier Vet Mic 2010; 145: 334-338.
    PubMedGoogle Scholar
  • 22 Osterhaus AD, Drost GA, Wirahadiredja RM, van den Ingh TS. Canine viral enteritis: prevalence of parvo-, corona- and rotavirus infections in dogs in the Netherlands. Vet Q 1980; 2: 181-190.
    CrossrefPubMedGoogle Scholar
  • 23 Parrish CR, O’Connell PH, Evermann JF, Carmichael LE. Natural variation of canine parvovirus. Science 1985; 230: 1046-1048.
    CrossrefPubMedGoogle Scholar
  • 24 Pratelli A, Buonavoglia D, Tempesta M, Guarda F, Carmichael L, Buonavoglia C. Fatal canine parvovirus type-1 infection in pubs from Italy. J Vet Invest 1999; 11: 365-367.
    PubMedGoogle Scholar
  • 25 Yesilbag K, Yilmaz Z, Özkul A, Pratelli A. Aetiological role of viruses in puppies with diarrhea. Vet Rec 2007; 161: 169-170.
    CrossrefPubMedGoogle Scholar