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Tierarztl Prax Ausg K Kleintiere Heimtiere 2017; 45(05): 295-300
DOI: 10.15654/TPK-170058
Originalartikel
Schattauer GmbH

Heterogenität der kaninen Immunantworten gegen Borrelia burgdorferi in einem Line-Immunoassay mit rekombinantem VlsE und C6-Peptid

Heterogeneity of canine immune responses to Borrelia burgdorferi in a line immunoassay comprising recombinant VlsE and C6 peptide
Doris Breu
1   LABOKLIN GmbH & Co KG
,
Elisabeth Müller
1   LABOKLIN GmbH & Co KG
› Author Affiliations
Further Information

Publication History

Eingegangen: 26 January 2017

Akzeptiert nach Revision: 25 April 2017

Publication Date:
09 January 2018 (online)

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Zusammenfassung

Gegenstand: In einem Line-Immunoassay mit sieben Antigenen sowie rekombinantem VlsE (variable major protein like sequence, expressed) und synthetischem C6-Peptid wurde die Präsenz Borreliaburgdorferi-spezifischer Antikörper (IgG) in kaninen Seren erfasst. Ziel der Studie war, die Antikörperantworten gegen das C6-Peptid und das VlsE-Protein zu vergleichen und zu evaluieren, ob diese divergieren, da Antikörper gegen die jeweiligen Antigene als spezifisch für eine stattgefundene Infektion mit Borrelien gelten. Material und Methoden: Es wurden 1355 Blutproben von Hunden analysiert, bei denen Verdacht auf eine Infektion mit Borrelien bestand. Die Testung erfolgte mittels eines Line-Immunoassays (IgG) mit neun Antigenen. Ergebnisse: Insgesamt 64,4% der Proben wurden als negativ bewertet, 16,4% als infiziert und 17,4% zeigten ein für eine Impfung spezifisches Bandenmuster. Bei 1,2% der Hunde lag sowohl eine Impfung als auch eine Infektion vor. Die am häufigsten positiv getesteten Banden waren p100 (24,3%), p31/OspA (18,5%), C6 (16,3%) und VlsE (13,9%). Bei 236 Hunden wurden Antikörper gegen das VlsE-Protein und/oder das C6-Peptid nachgewiesen. Während 73,3% dieser Hunde übereinstimmende Ergebnisse zwischen VlsE und C6 aufwiesen, unterschieden sich bei 26,7% die Resultate. Schlussfolgerung und klinische Relevanz: Die Testung mittels Line-Immunoassay erlaubt eine qualitative Analyse der unterschiedlichen IgG-Immunantworten gegen verschiedene Antigene. Die Resultate der Immunantworten gegen die als infektionsspezifisch geltenden Antigene VlsE und C6 divergierten bei mehr als 26% der positiv getesteten Hunde unserer Population, sodass zum Ausschluss oder der Bestätigung einer Infektion mit Borrelia burgdorferi möglichst unterschiedliche infektionsspezifische Banden berücksichtigt werden sollten.

Summary

Objective: The study aimed to investigate the distribution of specific immune responses (IgG) to Borrelia burgdorferi using a line immunoassay with recombinant VlsE (variable major protein-like sequence, expressed) protein and synthetic C peptide among other antigens. We compared the immune responses to VlsE protein and C6 peptide, because both antigens have been considered specific for a Borrelia infection. Materials and methods: A total of 1355 blood samples from dogs suspected of Borrelia infection were analysed. The line immunoassay employed nine antigens. Results: A total of 64.4% of all samples tested negative, 16.4% were positive for an infection and 17.4% were positive for vaccination. Band patterns specific for both infection and vaccination were observed in 1.2% of the dogs. The bands that most frequently tested positive were p100 (24.3%), p31/OspA (18.5%), C6 (16.3%) and VlsE (13.9%). A total of 236 dogs (17.4% of the population) had antibodies to VlsE and/or C6 peptide. In 73.3% of these dogs, results for VlsE and C6 peptide were consistent, whereas this was not the case for 26.7% of these animals. Conclusion and clinical relevance: Testing using a line immunoassay allows for qualitative analyses of different immune responses to various antigens used as probes. In our study, > 26% of the dogs displayed discrepant results with regard to VlsE and C6, the two antigens considered specific for Borrelia burgdorferi infection. To confirm or rule out infection, the results of several band patterns, thought to be specific for infection, need to be taken into consideration.

Schlüsselwörter

Hund - Infektionsmedizin - Labordiagnostik - Borrelien - Western Blot

Keywords

Dog - infectious medicine - laboratory diagnostics - Western blot
 

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