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CC BY-NC-ND 4.0 · Arq Neuropsiquiatr 2016; 74(09): 730-736
DOI: 10.1590/0004-282X20160108
ARTICLES

Propentofylline reduces glial scar development following gliotoxic damage in the rat brainstem

Propentofilina reduz o desenvolvimento da cicatriz glial após dano gliotóxico no tronco encefálico de ratos
Eduardo Fernandes Bondan
1   Universidade Paulista, Departamento de Patologia Ambiental e Experimental, São Paulo SP, Brasil;
2   Universidade Cruzeiro do Sul, Departamento de Medicina Veterinária, São Paulo SP, Brasil.
,
Maria de Fátima Monteiro Martins
1   Universidade Paulista, Departamento de Patologia Ambiental e Experimental, São Paulo SP, Brasil;
2   Universidade Cruzeiro do Sul, Departamento de Medicina Veterinária, São Paulo SP, Brasil.
,
Pietro Domingues Dossa
1   Universidade Paulista, Departamento de Patologia Ambiental e Experimental, São Paulo SP, Brasil;
,
Lígia Bocamino Viebig
1   Universidade Paulista, Departamento de Patologia Ambiental e Experimental, São Paulo SP, Brasil;
,
Carolina Vieira Cardoso
1   Universidade Paulista, Departamento de Patologia Ambiental e Experimental, São Paulo SP, Brasil;
,
João Lopes Martins Júnior
1   Universidade Paulista, Departamento de Patologia Ambiental e Experimental, São Paulo SP, Brasil;
,
Maria Martha Bernardi
1   Universidade Paulista, Departamento de Patologia Ambiental e Experimental, São Paulo SP, Brasil;
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ABSTRACT

Propentofylline is a xanthine derivative that depresses activation of glial cells, whose responses contribute to neural tissue damage during inflammation. Ethidium bromide injection into the central nervous system induces local oligodendroglial and astrocytic loss, resulting in primary demyelination, neuroinflammation and blood-brain barrier disruption. Surviving astrocytes present a vigorous reaction around the injury site with increased immunoreactivity to glial fibrillary acidic protein (GFAP).

Objective This study aimed to evaluate the effect of propentofylline administration on astrocytic response following gliotoxic injury.

Method Wistar rats were injected with ethidium bromide into the cisterna pontis and treated or not with propentofylline (12.5mg/kg/day, intraperitoneal) during the experimental period. Brainstem sections were collected from 15 to 31 days after gliotoxic injection and processed for GFAP immunohistochemistry.

Results and Conclusion Results demonstrate that propentofylline decreased astrocytic activation until the 21st day, suggesting that this drug may have a role in reducing glial scar development following injury.

RESUMO

A propentofilina é uma xantina que deprime a ativação das células gliais, cujas respostas contribuem para o dano neural durante inflamação. A injeção de brometo de etídio no sistema nervoso central induz a perda oligodendroglial e astrocitária, resultando em desmielinização, neuroinflamação e ruptura da barreira hematoencefálica. Os astrócitos sobreviventes apresentam vigorosa reação ao redor da lesão com aumento da imunorreatividade à proteína glial fibrilar ácida (GFAP).

Objetivo Este estudo objetivou avaliar o efeito da propentofilina sobre a resposta astrocitária após injúria gliotóxica.

Método Ratos Wistar foram injetados com brometo de etídio na cisterna basal e tratados ou não com propentofilina (12.5mg/kg/dia, intraperitoneal). Amostras do tronco encefálico foram coletadas dos 15 aos 31 dias pós-injeção do gliotóxico e processadas para estudo ultraestrutural e imuno-histoquímico para GFAP.

Resultados e Conclusão Os resultados demonstram que a propentofilina reduziu a ativação astrocitária até o 21o dia, sugerindo que essa droga pode atuar na redução da cicatriz glial após injúria.

astrocytes - ethidium - gliosis - gliotoxin - xanthine
astrócitos - etídio - gliose - gliotoxina - xantina


Publikationsverlauf

Eingereicht: 29. Februar 2016

Angenommen: 08. Juni 2016

Artikel online veröffentlicht:
06. September 2023

© 2023. Academia Brasileira de Neurologia. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commecial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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