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CC BY-NC-ND 4.0 · Arq Neuropsiquiatr 2017; 75(02): 96-102
DOI: 10.1590/0004-282X20160194
ARTICLES

Experimental neurocysticercosis: absence of IL-4 induces lower encephalitis

Neurocisticercose experimental: ausência de IL-4 induz menos encefalite
Hidelberto Matos Silva
1   Universidade Federal de Goiás, Instituto de Patologia Tropical e Saúde Pública, Goiânia GO, Brasil;
2   Centro Universitário UNIRG, Faculdade de Medicina, Gurupi TO, Brasil.
,
Marina Clare Vinaud
1   Universidade Federal de Goiás, Instituto de Patologia Tropical e Saúde Pública, Goiânia GO, Brasil;
,
Ruy de Souza Lino Júnior
1   Universidade Federal de Goiás, Instituto de Patologia Tropical e Saúde Pública, Goiânia GO, Brasil;
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ABSTRACT

Neurocysticercosis (NCC) is the most severe clinical manifestation of cysticercosis. One of the factors responsible for its symptomatology is the host inflammatory response. Therefore the influence of interleukin 4 (IL-4) on the induction of encephalitis in experimental NCC was evaluated.

Methods

BALB/c (WT) and BALB/c (IL-4-KO) mice were inoculated intracranially with Taenia crassiceps cysticerci and euthanized at 7, 30, 60 and 90 days later, the encephala removed and histopathologically analyzed.

Results

The absence of IL-4 induced greater parasitism. In the initial phase of the infection, IL-4-KO showed a lower intensity in the inflammatory infiltration of polimorphonuclear cells in the host-parasite interface and intra-parenquimatous edema. The IL-4-KO animals, in the late phase of the infection, showed lower intensity of ventriculomegaly, encephalitis, and meningitis, and greater survival of the parasites in comparison with the WT animals.

Conclusion

The absence of IL-4 induced lower inflammatory infiltration, ventriculomegaly and perivasculitis in experimental NCC.

RESUMO

A Neurocisticercose (NCC) é a manifestação clínica mais severa da cisticercose, e um dos fatores responsáveis pela sintomatologia é a resposta inflamatória do hospedeiro. Desta forma avaliou-se a influência da interleucina 4 (IL-4) na indução de encefalite na NCC experimental.

Métodos

Camundongos das linhagens BALB/c (WT) e BALB/c (IL-4-KO) foram inoculados intracranialmente com cisticercos de Taenia crassiceps e eutanasiados aos 7, 30, 60 e 90 dias após a infecção, os encéfalos foram removidos e analisados histopatologicamente.

Resultados

A ausência da IL-4 induziu um maior parasitismo nos animais. Na fase inicial da infecção os animais IL-4-KO apresentaram menor intensidade tanto de infiltrado inflamatório de polimorfonucleares na interface parasito-hospedeiro quanto de edema intraparenquimatoso. Os animais IL-4-KO, na fase tardia, apresentaram menor intensidade de ventriculomegalia, encefalite, meningite e maior sobrevivência dos cisticercos em relação aos animais WT.

Conclusão

A ausência da IL-4 induz menos infiltrado inflamatório, ventriculomegalia e perivasculite na NCC experimental.

neurocysticercosis - Taenia crassiceps - encephalitis - interleukin-4
neurocisticercose - Taenia crassiceps - encefalite - interleukin-4


Publication History

Received: 02 May 2016

Accepted: 31 October 2016

Article published online:
05 September 2023

© 2023. Academia Brasileira de Neurologia. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commecial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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