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CC BY-NC-ND 4.0 · Arq Neuropsiquiatr 2017; 75(08): 546-552
DOI: 10.1590/0004-282X20170092
ARTICLES

Curcumin decreases astrocytic reaction after gliotoxic injury in the rat brainstem

Curcumina reduz a reação astrocitária após injúria gliotóxica no tronco encefálico de ratos
Eduardo Bondan
1   Universidade Paulista, Patologia Ambiental e Experimental, São Paulo SP, Brasil;
2   Universidade Cruzeiro do Sul, Medicina Veterinária, São Paulo SP, Brasil.
,
Carolina Cardoso
1   Universidade Paulista, Patologia Ambiental e Experimental, São Paulo SP, Brasil;
,
Maria de Fátima Martins
1   Universidade Paulista, Patologia Ambiental e Experimental, São Paulo SP, Brasil;
2   Universidade Cruzeiro do Sul, Medicina Veterinária, São Paulo SP, Brasil.
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ABSTRACT

Recent studies have demonstrated that curcumin (Cur) has antioxidant, anti-inflammatory and anti-fibrotic effects. Ethidium bromide (EB) injections into the central nervous system (CNS) are known to induce local oligodendroglial and astrocytic loss, resulting in primary demyelination and neuroinflammation. Peripheral astrogliosis is seen around the injury site with increased immunoreactivity to glial fibrillary acidic protein (GFAP). This investigation aimed to evaluate the effect of Cur administration on astrocytic response following gliotoxic injury. Wistar rats were injected with EB into the cisterna pontis and treated, or not, with Cur (100 mg/kg/day, intraperitoneal route) during the experimental period. Brainstem sections were collected at 15, 21 and 31 days after EB injection and processed for GFAP immunohistochemical staining. Astrocytic reactivity was measured in a computerized system for image analysis. In Cur-treated rats, the GFAP-stained area around the lesion was significantly smaller in all periods after EB injection compared to untreated animals, showing that Cur reduces glial scar development following injury.

RESUMO

Estudos recentes têm demonstrado que a curcumina (Cur) possui efeitos antioxidantes, anti-inflamatórios e antifibróticos. Sabe-se que a injeção de brometo de etídio (EB) no sistema nervoso central induz a perda oligodendroglial e astrocitária, resultando em desmielinização primária e neuroinflamação. Astrogliose periférica é observada ao redor da lesão com aumento da imunorreatividade à proteína glial fibrilar ácida (GFAP). A presente investigação objetivou avaliar o efeito da Cur sobre a resposta astrocitária após injúria gliotóxica. Ratos Wistar foram injetados com EB na cisterna basal e tratados ou não com Cur (100 mg/kg/dia, via intraperitoneal) durante o período experimental. Amostras do tronco encefálico foram coletadas aos 15, 21 e 31 dias pós-injeção de EB e processadas para estudo imuno-histoquímico para a GFAP. A reatividade astrocitária foi medida em um sistema computadorizado para análise de imagem. Nos ratos tratados com Cur, a área marcada para GFAP foi significantemente menor em todos os períodos pós-injeção de EB, indicando que a Cur reduz o desenvolvimento da cicatriz glial após injúria.

astrocytes - curcumin - ethidium - gliosis - gliotoxin
astrócitos - curcumina - etídio - gliose - gliotoxina


Publikationsverlauf

Eingereicht: 21. Dezember 2016

Angenommen: 04. Mai 2017

Artikel online veröffentlicht:
05. September 2023

© 2023. Academia Brasileira de Neurologia. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commecial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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