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DOI: 10.3233/PGE-13042
Application of real-time polymerase chain reaction in the clinical genetic practice
Verantwortlicher Herausgeber dieser Rubrik:
Publikationsverlauf
05. Dezember 2012
07. März 2013
Publikationsdatum:
27. Juli 2015 (online)
![](https://www.thieme-connect.de/media/10.1055-s-00029027/201301/lookinside/thumbnails/10.3233-pge-13042-1.jpg)
Abstract
The development of polymerase chain reaction revolutionized the molecular genetics and diagnostics. Technical improvements helped to make more specific and sensitive target determinations. Introduction of real-time polymerase chain reaction makes possible several applications in clinical genetics like detection of gene mutations, single nucleotide polymorphisms, deletions, measurement of gene expressions, micro ribonucleic acids, free nucleic acids and microbial genomes. Here I discuss a few examples for specific applications in prenatal clinical genetic practice. These are the detection of microbial genomes, deletions, trisomies, mutations, single nucleotide polymorphisms and free nucleic acids.