1H NMR investigation of normal and osteoarthritic synovial fluid in the horse

L. Lacitignola; F. P. Fanizzi; E. Francioso; A. Crovace

doi:10.3415/VCOT-06-12-0101

Veterinary and Comparative Orthopaedics and Traumatology, Table of Contents

Vet Comp Orthop Traumatol 2008; 21(01): 85-88
DOI: 10.3415/VCOT-06-12-0101

Clinical Communication

Schattauer GmbH

^[1]1H NMR investigation of normal and osteoarthritic synovial fluid in the horse

L. Lacitignola

¹Dipartimento di Scienze Cliniche Veterinarie, Facoltà di Medicina Veterinaria, Università degli studi di Teramo, Teramo, Italy

,

F. P. Fanizzi

³Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Università di Lecce, Lecce, and Consortium C.A.R.S.O. Cancer Research Center, Bari, Italy

,

E. Francioso

²D.E.T.O. Veterinary Surgery,University of Bari, Bari, Italy

,

A. Crovace

²D.E.T.O. Veterinary Surgery,University of Bari, Bari, Italy

› Author Affiliations

Abstract

Summary

Proton magnetic resonance spectroscopy (¹H NMR) has been successfully used in the study of many biological fluids. The data presented here report on the metabolic profiles of normal equine synovial fluids compared with osteoarthritic (OA) fluids. Twenty-five OA synovial fluid samples and eight normal ones were collected from the forelimb fetlock joint in 22 horses, aged between five and 24 years. ¹H NMR spectroscopy was carried out with a Bruker Avance DRX 500 equiped with a cryomagnet working at 11 Tesla, and ‘Mestre-C 4.9.9.6’ software was used to analyze the spectra. The study assessed the increase of lactate, alanine, acetate, N-acetylglucosamine, pyruvate, citrate, creatine/creatinine, glycerol, HDL choline, and α-glucose in OA synovial fluid. The variations observed in samples from horses with OA compared to those in the control group, and similar data found in other studies, confirm that this technique may be useful in the study of joint metabolism. Its practical application may be in the evaluation of the treatment of OA in athletic horses.

Keywords

Osteoarthritis - synovial fluid - nucler magnetic resonance spectroscopy - equine

Full Text

References

References
1 Claxson A, Grootveld M, Chander C. et al. Examination of the metabolic status of rat air pouch inflammatory exudate by high field proton NMR spectroscopy. BiochimBiophys Acta 1999; 1454: 57-70.
2 Nicholson JK, O'Flynn MP, Sadler PJ. et al. Proton-nuclear-magnetic-resonance studies of serum, plasma and urine from fasting normal and diabetic subjects. BiochemJ 1984; 217: 365-375.
3 Williamson MP, Humm G, Crisp AJ. 1Hnuclearmag- netic resonance investigation of synovial fluid components in osteoarthritis, rheumatoid arthritis and traumatic effusions. Br J Rheumatol 1989; 28: 23-27.
4 Braun S, Kalinowski HO, Berger S. 150 and More Basic NMR experiments. WILE-VCH. 1998
5 Duffy JM, Grimshaw J, Guthrie DJ. et al. 1H-nuclear magnetic resonance studies of human synovial fluid in arthritic disease states as an aid to confirming metabolic activity in the synovial cavity. Clin Sci (Lond) 1993; 85: 343-351.
6 Grootveld M, Henderson EB, Farrell A. et al. Oxidative damage to hyaluronate and glucose in synovial fluid during exercise of the inflamed rheumatoid joint. Detection of abnormal low-molecular-mass metabolites by proton-n.m.r. spectroscopy. Biochem J 1991; 273 (Pt 2) 459-467.
7 Meshitsuka S, Yamazaki E, Inoue M. et al. Nuclear magnetic resonance studies of synovial fluids from patients with rheumatoid arthritis and osteoarthritis. Clin Chim Acta 1999; 281: 163-167.
8 Naughton DP, Haywood R, Blake DR. et al. A comparative evaluation of the metabolic profiles of normal and inflammatory knee-joint synovial fluids by high resolution proton NMR spectro- scopy. FEBS Lett 1993; 332: 221-225.
9 Schiller J, Arnhold J, Sonntag K. et al. NMR studies on human, pathologically changed synovial fluids: role of hypochlorous acid. Magn Reson Med 1996; 35: 848-853.
10 Silwood CJ, Chikanza IC, Tanner KE. et al. Investigation of the molecular nature of low-molecular- mass cobalt(II) ions in isolated osteoarthritic knee-joint synovial fluid. Free Radic Res 2004; 38: 561-571.
11 Silwood Silwood, Grootveld M. Evaluation of the speci- ation status of aluminium(III) ions in isolated osteoarthritic knee-joint synovial fluid. Biochim Biophys Acta 2005; 1725: 327-339.
12 Silwood Silwood, Grootveld M. Chemical nature of implant-derived titanium(IV) ions in synovial fluid. Biochem Biophys Res Commun 2005; 330: 784-790.
13 Silwood Silwood, Grootveld M. Examination of the molecular nature of low-molecular-mass chro- mium(III) ions in isolated osteoarthritic knee- joint synovial fluid. J Inorg Biochem 2005; 99: 1390-1400.
14 Crovace A, Lacitignola L, Miolo A. Surgery plus chondroprotection for canine cranial cruciate ligament (CCL) rupture: A proton-NMR study. (Erratum Vet Comp Orthop Traumatol 2007;20:149) Vet Comp Orthop Traumatol 2006; 19: 239-245.
15 Damyanovich AZ, Staples JR, Marshall KW. !H NMR investigation of changes in the metabolic profile of synovial fluid in bilateral canine os- teoarthritis with unilateral joint denervation. Osteoarthritis Cartilage 1999; 7: 165-172.
16 Damyanovich AZ, Staples JR, Chan AD. et al. Comparative study of normal and osteoarthritic canine synovial fluid using 500 MHz 1H magnetic resonance spectroscopy. JOrthop Res 1999; 17: 223-231.
17 Marshall KW, Manolopoulos V, Mancer K. et al. Amelioration of disease severity by intraarticular hylan therapy in bilateral canine osteoarthritis. J Orthop Res 2000; 18: 416-425.
18 Damyanovich AZ, Staples J, Marshall KW. A comparative study of deproteinized human synovial fluid in early and latestage osteoarthritis using 500 MHz 'H-NMR spectroscopy. Proceedings of International Society for Magnetic Resonance in Medicine. April 12 1997
19 Naughton D, Whelan M, Smith EC. et al. An investigation of the abnormal metabolic status of synovial fluid from patients with rheumatoid arthritis by high field proton nuclear magnetic resonance spectroscopy. FEBS Lett 1993; 317: 135-138.
20 Palmer Palmer, Bertone AL. Joint Biomechanics in the pathogenesis of traumatic arthritis. In: McIlwraith WC. (ed). Joint disease in the horse. Philadelphia: W.B. Saunders; 1996. pp 104-119.
21 Geborek P, Moritz U, Wollheim FA. Joint capsular stiffness in knee arthritis. Relationship to intraar- ticular volume, hydrostatic pressures, and extensor muscle function. J Rheumatol 1989; 16: 1351-1358.
22 Damyanovich AZ, Staples JR, Marshall KW. The effects of freeze/thawing on human synovial fluid observed by 500 MHz 1H magnetic resonance spectroscopy. J Rheumatol 2000; 27: 746-752.
23 McIlwraith WC. General pathobiology ofthejoint and response to injury. In: McIlwraith WC. (ed). Joint disease in the horse. Philadelphia: WB. Saunders; 1996. pp 104-119.
24 Schiller J, Arnhold J, Schwinn J. et al. Reactivity of cartilage and selected carbohydrates with hydro- xyl radicals: an NMR study to detect degradation products. Free Radic Res 1998; 28: 215-228.
25 Grootveld M, Silwood CJ, Lynch EJ. et al. The role of N-acetylcysteine in protecting synovial fluid biomolecules against radiolytically-mediated ox- idative damage: a high field proton NMR study. Free Radic Res 1999; 30: 351-369.
26 Schiller J, Arnhold J, Arnold K. NMR studies of the action of hypochlorous acid on native pig articular cartilage. Eur J Biochem 1995; 233: 672-676.
27 Schiller J, Arnhold J, Zachaus A. et al. Reaction of hypochlorous acid with bovine nasal cartilage comparison to pig articular cartilage. Z Naturforsch 1997; [C ] 52 694-701.