Chondrogenic potential of mesenchymal stromal cells derived from equine bone marrow and umbilical cord blood

L. C. Berg; T. G. Koch; T. Heerkens; K. Besonov; P. D. Thomsen; D. H. Betts

doi:10.3415/VCOT-08-10-0107

Veterinary and Comparative Orthopaedics and Traumatology, Table of Contents

Vet Comp Orthop Traumatol 2009; 22(05): 363-370
DOI: 10.3415/VCOT-08-10-0107

Original Research

Schattauer GmbH

Chondrogenic potential of mesenchymal stromal cells derived from equine bone marrow and umbilical cord blood

Authors

L. C. Berg

¹Department of Basic Animal and Veterinary Sciences, Faculty of Life Sciences, University of Copenhagen, Frederiksberg C, Denmark
T. G. Koch

¹Department of Basic Animal and Veterinary Sciences, Faculty of Life Sciences, University of Copenhagen, Frederiksberg C, Denmark

²Department of Biomedical Sciences, University of Guelph, Guelph, Canada
T. Heerkens

²Department of Biomedical Sciences, University of Guelph, Guelph, Canada
K. Besonov

²Department of Biomedical Sciences, University of Guelph, Guelph, Canada
P. D. Thomsen

¹Department of Basic Animal and Veterinary Sciences, Faculty of Life Sciences, University of Copenhagen, Frederiksberg C, Denmark
D. H. Betts

²Department of Biomedical Sciences, University of Guelph, Guelph, Canada

Abstract

Summary

Objective: Orthopaedic injury is the most common cause of lost training days or premature retirement in the equine athlete. Cell-based therapies are a potential new treatment option in musculo-skeletal diseases. Mesenchymal stromal cells (MSC) have been derived from multiple sources in the horse including bone marrow and umbilical cord blood. The objective of this study was to provide an in vitro comparison of the chondrogenic potential in MSC derived from adult bone marrow (BM-MSC) and umbilical cord blood (CB-MSC).

Results: MSC from both sources produced tissue with cartilage-like morphology that stained positive for proteoglycans and expressed cartilage markers. The CB-MSC pellets were larger and showed hyaline-like cartilage morphology as early as day six. Gene expression of collagen type 21, aggrecan and CD-RAP was higher in CB- than BM-MSC pel-lets. Expression of Sox9 mRNA was similar between CB- and BM-MSC pellets. Protein concentration of cartilage-derived retinoic acid sensitive protein was higher in culture medium from CB- than BM-MSC pellets.

Conclusion: CB-MSC and BM-MSC were both capable of producing hyaline-like cartilage in vitro. However, in this study the MSC from umbilical cord blood appeared to have more chondrogenic potential than the BMMSC based on the cells tested and parameters measured.

Supplementary Information for this paper is available on the VCOT website at www.VCOTonline.com.

Keywords

Chondrogenesis - mesenchymal stromal cells (MSC) - bone marrow - cord blood - horse

Full Text

References

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