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Vet Comp Orthop Traumatol 2009; 22(03): 216-221
DOI: 10.3415/VCOT08-02-0021
Original Research
Schattauer GmbH

Cartilage replacement in dogs

A preliminary investigation of colonization of ceramic matrices
G. Hauschild
1   University Hospital of Münster, Department of Orthopedics, Germany
2   Clinic for Small Domestic Animals, University of Veterinary Medicine Hannover, Foundation, Germany
3   Kleintierklinik Menzel, Recklinghausen, Germany
,
N. Muschter
2   Clinic for Small Domestic Animals, University of Veterinary Medicine Hannover, Foundation, Germany
,
A. Richter
4   Center for Human Genetics, University of Bremen, Germany
,
H. Ahrens
1   University Hospital of Münster, Department of Orthopedics, Germany
,
G. Gosheger
1   University Hospital of Münster, Department of Orthopedics, Germany
,
M. Fehr
2   Clinic for Small Domestic Animals, University of Veterinary Medicine Hannover, Foundation, Germany
,
J. Bullerdiek
4   Center for Human Genetics, University of Bremen, Germany
› Institutsangaben
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Publikationsverlauf

Received:25. Februar 2008

Accepted:03. März 2008

Publikationsdatum:
17. Dezember 2017 (online)

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Summary

The objective of this study was to examine the behaviour of canine chondrocytes following colonisation of a β-tricalcium phosphate (β-TCP, Cerasorb®, Curasan) matrix. In total, five of these cylinders were inoculated with 1.5 ml of cell suspension and subsequently incubated for about one week. In the second part of the experiment, another five Cerasorb® cylinders were each studded with two cartilage chips of variable size and then incubated for about one week. The series of experiments were analyzed using cell staining and imaging techniques that included scanning electron microscopy. Cell migration onto the matrix was proven for both colonization methods. It was observed that colonising the cylinders by pipetting cell suspension on them produced far better results, with respect to both growth rate and spreading of the cells, than did colonisation by studding with cartilage chips. A homogenous, surface-covering colonisation with predominantly living cells was demonstrated by scanning electron microscopy in the chondrocyte morphology. In comparison to cell-culture controls, there was a clearly better colonisation, with cells attached to both the material's primary grains and its micropores. The ceramic studied is well accepted by canine chondrocytes, and appears to be fundamentally well-suited as a matrix for bio-artificial bone-cartilage replacement. Additional qualitative analyses and a series of experiments aiming to accelerate cell proliferation are planned for subsequent studies.

Keywords

Cartilage replacement - bioartificial graft - matrix
 

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