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DOI: 10.4103/0971-5851.171541
Emergence of micronuclei as a genomic biomarker
![](https://www.thieme-connect.de/media/10.1055-s-00050348/201504/lookinside/thumbnails/10_4103_0971-5851_171541_212-1.jpg)
Abstract
The presence of micronuclei (MN) in mammalian cells is related to several mutagenetic stresses. MN are formed as a result of chromosome damage and can be readily identified in exfoliated epithelial cells. MN is chromatin particles derived from acentric chromosomal fragments, which are not incorporated into the daughter nucleus after mitosis. It can be visualized by chromatin stains. A variety of factors influences the formation of MN in cells such as age, sex, genetic constitution, physical and chemical agents, adverse habits such as tobacco, areca nut chewing, smoking, and alcohol consumption. Micronucleation has important implications in the genomic plasticity of tumor cells. The present paper reviews the origin, fate and scoring criteria of MN that serves as a biomarker of exposure to genetic toxins, and for the risk of cancer.
Publication History
Article published online:
12 July 2021
© 2015. Indian Society of Medical and Paediatric Oncology. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/.)
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References
-
1 Alexandrescu I, Havarneanu D, Popa D. New approaches in biomonitring human populations exposed to genotoxic agents: Epithelial cell micronucleus assay. J Preventive Med 2006;14:57-65.
-
2 Veerachari U, Venkatesh S, Yadav A, Narayanappa R. Biomonitring genetic instability in normal healthy population using a simple cytogenetic marker-micronucleus test. Int Multidiscip Res J 2011;1:1-9.
-
3 Sivasankari NP, Reddy KS, Kaur S, Vivekanandam S, Rao KR. Micronucleus index: An early diagnosis in oral carcinoma. J Anat Soc India 2008;57:8-13.
-
4 Sellappa S, Balakrishnan M, Raman S, Palanisamy S. Induction of micronuclei in buccal mucosa on chewing a mixture of betel leaf, areca nut and tobacco. J Oral Sci 2009;51:289-92.
-
5 Unal M, Celik A, Ates NA, Micozkadioglu D, Derici E, Pata YS, et al. Cytogenetic biomonitoring in children with chronic tonsillitis: Micronucleus frequency in exfoliated buccal epithelium cells. Int J Pediatr Otorhinolaryngol 2005;69:1483-8.
-
6 Roth JM, Restani RG, Gonçalves TT, Sphor SL, Ness AB, Martino-Roth MG, et al. Genotoxicity evaluation in chronic renal patients undergoing hemodialysis and peritoneal dialysis, using the micronucleus test. Genet Mol Res 2008;7:433-43.
-
7 Ramos-Remus C, Dorazco-Barragan G, Aceves-Avila FJ, Alcaraz-Lopez F, Fuentes-Ramirez F, Michel-Diaz J, et al. Genotoxicity assessment using micronuclei assay in rheumatoid arthritis patients. Clin Exp Rheumatol 2002;20:208-12.
-
8 Kumar V, Rao NN, Nair NS. Micronuclei in oral squamous cell carcinoma. A marker of genotoxic damage. Indian J Dent Res 2000;11:101-6.
-
9 Kamboj M, Mahajan S. Micronucleus - an upcoming marker of genotoxic damage. Clin Oral Investig 2007;11:121-6.
-
10 Buajeeb W, Kraivaphan P, Amornchat C, Suthamajariya K. Reduction of micronuclei in oral lichen planus supplemented with beta-carotene. J Oral Sci 2008;50:461-7.
-
11 Jois HS, Kale AD, Mohan Kumar KP. Micronucleus as potential biomarker of oral carcinogenesis. Indian J Dent Adv 2010;2:197-202.
-
12 Thomas P, Holland N, Bolognesi C, Kirsch-Volders M, Bonassi S, Zeiger E, et al. Buccal micronucleus cytome assay. Nat Protoc 2009;4:825-37.
-
13 Palaskar S, Jindal C. Evaluation of micronuclei using papanicolaou and May Grunwald Giemsa stain in individuals with different tobacco habits - A comparative study. J Clin Diagn Res 2010;4:3607-13.
-
14 Torres-Bugarín O, Covarrubias-Bugarín R, Zamora-Perez AL, Torres-Mendoza BM, García-Ulloa M, Martínez-Sandoval FG. Anabolic androgenic steroids induce micronuclei in buccal mucosa cells of bodybuilders. Br J Sports Med 2007;41:592-6.
-
15 Tinwell H, Ashby J. Micronucleus morphology as a means to distinguish aneugens and clastogens in the mouse bone marrow micronucleus assay. Mutagenesis 1991;6:193-8.
-
16 Utani K, Kohno Y, Okamoto A, Shimizu N. Emergence of micronuclei and their effects on the fate of cells under replication stress. PLoS One 2010;5:e10089.
-
17 Holland N, Bolognesi C, Kirsch-Volders M, Bonassi S, Zeiger E, Knasmueller S, et al. The micronucleus assay in human buccal cells as a tool for biomonitoring DNA damage: The HUMN project perspective on current status and knowledge gaps. Mutat Res 2008;659:93-108.
-
18 Heddle SM, JA. Short-Term Tests for Chemical Carcinogens. In: Stich SR, editor. The Micronucleus Assay. I. In vivo. Berlin/New York: Springer-Verlag; 1981. p. 243-9.
-
19 Tolbert PE, Shy CM, Allen JW. Micronuclei and other nuclear anomalies in buccal smears: Methods development. Mutat Res 1992;271:69-77.
-
20 Tolbert PE, Shy CM, Allen JW. Micronuclei and other nuclear anomalies in buccal smears: A field test in snuff users. Am J Epidemiol 1991;134:840-50.
-
21 Beliën JA, Copper MP, Braakhuis BJ, Snow GB, Baak JP. Standardization of counting micronuclei: Definition of a protocol to measure genotoxic damage in human exfoliated cells. Carcinogenesis 1995;16:2395-400.
-
22 Kashyap B, Reddy PS. Micronuclei assay of exfoliated oral buccal cells: Means to assess the nuclear abnormalities in different diseases. J Cancer Res Ther 2012;8:184-91.
-
23 Fenech M, Holland N, Chang WP, Zeiger E, Bonassi S. The human micronucleus project - An international collaborative study on the use of the micronucleus technique for measuring DNA damage in humans. Mutat Res 1999;428:271-83.
-
24 Chial H. Cytogenetic methods and disease: Flow cytometry, CGH, and FISH. Nat Educ 2008;1:76.
-
25 Avlasevich SL, Bryce SM, Cairns SE, Dertinger SD. In vitro micronucleus scoring by flow cytometry: Differential staining of micronuclei versus apoptotic and necrotic chromatin enhances assay reliability. Environ Mol Mutagen 2006;47:56-66.
-
26 Bryce SM, Bemis JC, Avlasevich SL, Dertinger SD. In vitro micronucleus assay scored by flow cytometry provides a comprehensive evaluation of cytogenetic damage and cytotoxicity. Mutat Res 2007;630:78-91.
-
27 Avlasevich S, Bryce S, De Boeck M, Elhajouji A, Van Goethem F, Lynch A, et al. Flow cytometric analysis of micronuclei in mammalian cell cultures: Past, present and future. Mutagenesis 2011;26:147-52.
-
28 Ramírez MJ, Surrallés J, Galofré P, Creus A, Marcos R. FISH analysis of 1cen-1q12 breakage, chromosome 1 numerical abnormalities and centromeric content of micronuclei in buccal cells from thyroid cancer and hyperthyroidism patients treated with radioactive iodine. Mutagenesis 1999;14:121-7.