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CC BY-NC-ND 4.0 · J Lab Physicians 2013; 5(02): 079-082
DOI: 10.4103/0974-2727.119845
Original Article

In silico Analysis of TCR Vβ7 of Two Patients with Type 1 Diabetes Mellitus

Jianwei Zhou
Clinic Laboratory, The Affiliated Hospital of Jining Medical College, Jining, Shandong Province, China
,
Cui Kong
Department of Cardiovascular Disease, The Affiliated Hospital of Jining Medical College, Jining, Shandong Province, China
,
Xiukui Wang
Department of Stomatology, The Affiliated Hospital of Jining Medical College, Jining, Shandong Province, China
,
Yinfeng Jia
Clinic Laboratory, The Affiliated Hospital of Jining Medical College, Jining, Shandong Province, China
,
Li Wang
Department of Pharmocology, The First People's Hospital, Jining, Shandong Province, China
,
Hong Chang
Clinic Laboratory, The Affiliated Hospital of Jining Medical College, Jining, Shandong Province, China
,
Lin Sun
Department of Endocrinology, The Affiliated Hospital of Jining Medical College, Jining, Shandong Province, China
› Author Affiliations Source of Support: Nil.
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ABSTRACT

Objective: To compare the sequences and crystal structures of variable region of beta chain 7 (Vβ7) of T cell receptor (TCR) of two patients with type 1 diabetes mellitus (T1DM).

Patients and Methods: The skewness of TCR Vβ7 of two T1DM patients were detected with real-time florescence quantitative polymerase chain reaction (FQ-PCR) and deoxyribonucleic acid (DNA) melting curve analysis technique followed by being sequenced, the crystal structures of them were simulated according to CPH models 2.0 Server, IMGT database, and RasMol 2 software.

Results: The whole sequences of TCR Vβ7 of T1DM patient-1 were "CASRTAGQYEQYFGPGTR", that of patient-2 were "CASRTAGQYEQFFGPGTR"; the only difference between them lied on the 12th amino acid. The crystal structures of Vβ7 of the two patients simulated with backbone model were rather similar, while that with sphere model were obviously different.

Conclusion: Although the TCR Vβ7 of the T1DM patients share the similar gene sequences, their crystal structures simulated with sphere model are different, and the mechanism needs further study.

Keywords

Beta chain - crystal structure - T cell receptor - type 1 diabetes mellitus - variable region


Publication History

Article published online:
07 April 2020

© 2013.

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