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CC BY-NC-ND 4.0 · J Lab Physicians 2015; 7(01): 011-016
DOI: 10.4103/0974-2727.154780
Original Article

A Leap above Friedewald Formula for Calculation of Low-Density Lipoprotein-Cholesterol

Reema Kapoor
Department of Biochemistry, Maulana Azad Medical College, New Delhi, India
,
Montosh Chakraborty
Department of Biochemistry, Hindu Rao Medical College, New Delhi, India
,
Navpreet Singh
Department of Community Medicine, Government Medical College and Hospital, Chandigarh, India
› Author Affiliations Source of Support: Nil.
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ABSTRACT

Purpose: The purpose was to compare the different calculated methods of low-density lipoprotein cholesterol (LDL-C) estimation and to determine which of them correlate best with the direct method.

Materials and Methods: The records of 480 samples for lipid profile were analyzed. Apart from the direct method, LDL-C was calculated by Friedewald low-density lipoprotein cholesterol method (F-LDL-C), modified Friedewald low-density lipoprotein cholesterol method (MF-LDL-C), and Anandaraja low-density lipoprotein cholesterol method (A-LDL-C). Paired t-test and Pearson correlation were evaluated between the different methods. Degree of agreement between the calculated methods and direct method was detected by Bland-Altman graphical plots.

Results: A strong correlation was found between all calculated LDL-C methods and direct low-density lipoprotein cholesterol method (D-LDL-C) assay, that is, F-LDL-C versus D-LDL-C = 0.94; A-LDL-C versus D-LDL-C = 0.93 and MF-LDL-C versus D-LDL-C = 0.95. No statistically significant difference was found between D-LDL-C and MF-LDL-C. Bland-Altman plot for MF-LDL-C showed minimal negative bias.

Conclusions: The study pointed out that MF-LDL-C correlated maximally with D-LDL-C estimation at all levels of triglycerides and MF-LDL-C can be used in place of D-LDL-C when the direct method cannot be afforded.

Key words

Anandaraja - Friedewald - low-density lipoprotein cholesterol - modified Friedewald


Publication History

Article published online:
19 April 2020

© 2015.

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