Phytochemical screening, antibacterial and cytotoxic activity of different fractions of xylocarpus mekongensis bark

Background: In Bangladesh, the tree Xylocarpus mekongensis (Lamk.) M. Roem. is used as the traditional medicine for the treatment of a number of ailments. Objectives: The present study was undertaken for antibacterial activity and cytotoxicity study of the methanol, ethyl acetate and chloroform extract of X. mekongensis bark. Materials and methods: Antimicrobial activity has been investigated against Vibrio cholera, Shigella flexneri, Shigella boydii, Salmonella typhi, Salmonella paratyphi and Staphylococcus aureus by disc diffusion and broth macro- dilution assay. For cytotoxicity test, methanol, acetate and chloroform extract of bark of X. mekongensis were subjected to brine shrimp lethality bioassay. Results: The zone of inhibition has been observed with almost all bacteria with some exceptions. Minimum inhibitory concentrations (MIC) of the extracts were found to be significant. The LC₅₀ values of these three extracts were found to be 1280, 320 and 320 μg/ml, respectively. Conclusions: Findings of the study justify the use of the plant in traditional medicine and suggests further investigation.

Key-words:

Xylocarpus mekongensis - Phytochemical screening - Antimicrobial activity - MIC - Brine shrimp lethality

Publikationsverlauf

Eingereicht: 31. Dezember 2012

Angenommen: 09. März 2013

Artikel online veröffentlicht:
07. Juli 2022