South Asian Journal of Cancer, Table of Contents CC BY-NC-ND 4.0 · South Asian J Cancer 2015; 04(03): 123-126DOI: 10.4103/2278-330X.173178 HEAD AND NECK CANCERS : Original Article An in vivo cytogenetic analysis of human oral squamous cell carcinoma Abhimanyu Mohanta Department of Zoology, Utkal University, Vani Vihar, Bhubaneshwar , Prafulla K. Mohanty Department of Zoology, Utkal University, Vani Vihar, Bhubaneshwar , Gadadhar Parida Department of Oncopathology, Acharya Harihar Regional Cancer Center, Cuttack, Odisha › Author Affiliations Recommend Article Abstract PDF Download Abstract Background: Oral cancer ranks in the top three of all cancers in India, which accounts for over 30% of all cancers reported in the country. The micronucleus test (MNT) is one of the most widely applied short term tests used in genetic toxicology to evaluate the mutagenicity and carcinogenicity. Aims: The present study aims at an in vivo cytogenetic analysis of human oral squamous cell carcinoma and to assess the applicability of MNT in diagnosing early detection of oral carcinoma. Materials and Methods: Exfoliated scrape smears were collected from the clinically diagnosed 136 patients suffering from oral precancerous and cancerous lesions. The wet fixed smears were stained by adopting Papanicolaou’s staining protocol and counter-stained with Giemsa’s solution. Results: The frequency of micronucleated cells has been observed to be in increasing order with the increase of the age-groups and from control to precancerous to cancerous cases significantly in both sexes. Conclusion: Micronucleus formation in the oral mucosa could be a biomarker of genetic damage and also a potential onco-indicator in the long run of oral carcinogenesis. Therefore, MNT can be applied for the early detection of oral carcinoma in the human being. Key words Key wordsExfoliated scrape smear - micronucleated cell - micronucleus test - precancerous and cancerous epithelium - nuclear entropy - carcinogenicity and pathogenicity PDF (558 kb) References References 1 Elango JK, Gangadharan P, Sumithra S, Kuriakose MA. Trends of head and neck cancers in urban and rural India. Asian Pac J Cancer Prev 2006; 7:108-12. 2 Coelho KR. Challenges of the oral cancer burden in India. J Cancer Epidemiol 2012; 2012:1-17. 3 Mehrotra R, Yadav S. Oral squamous cell carcinoma: Etiology, pathogenesis and prognostic value of genomic alterations. Ind J Cancer 2006; 43:60-6. 4 Stich HF, Rosin MP. Micronuclei in exfoliated human cells as a tool for studies in cancer risk and cancer intervention. Cancer Lett 1984; 22:241-53. 5 Halder A, Chakraborty T, Mandal K, Guru PK, Das S, Roychoudhury R, et al. Comparative study of exfoliated oral mucosal cells micronuclei frequency in normal, precancerous and malignant epithelium. 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